Offered Papers Theme A: Efficient Production from Grassland

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Harvesting and storing high quality forage in the cool humid regions remains a challenge due to the potential for protein degradation during ensiling. Red clover is an exception as high protein levels are maintained during ensiling. Decreased proteolytic activity in red clover is due to polyphenol oxidase (PPO) activity and appropriate o-diphenol substrates (Jones et al., 1995, Sullivan et al., 2004). This project was undertaken to determine if PPO activity is present in a range of grasses and the potential role in proteolytic inhibition in the presence of the o-diphenol caffeic acid.

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Polyphenol Oxidase Activity and in Vitro Proteolytic Inhibition in Grasses

Harvesting and storing high quality forage in the cool humid regions remains a challenge due to the potential for protein degradation during ensiling. Red clover is an exception as high protein levels are maintained during ensiling. Decreased proteolytic activity in red clover is due to polyphenol oxidase (PPO) activity and appropriate o-diphenol substrates (Jones et al., 1995, Sullivan et al., 2004). This project was undertaken to determine if PPO activity is present in a range of grasses and the potential role in proteolytic inhibition in the presence of the o-diphenol caffeic acid.