Satellite Symposium 5: Molecular Breeding
Description
Alfalfa (Medicago sativa L.) is the most important forage crop in Argentina, with ca. 6 million cultivated hectares. The production of this crop is limited by the alfalfa caterpillar (Colias lesbia) which causes a loss equivalent to at least 10% of the biomass per year. No natural tolerance against this lepidoptera was found in alfalfa germplasm, hampering the development of tolerant cultivars by conventional breeding. This pest is usually controlled by using chemical insecticides but this has adverse effects on beneficial insects and the environment. Alternatively, low doses of commercial Bt insecticides (40 to 70 g/ha) also proved to efficiently limit the pest. This observation leads to us consider that the development of alfalfa transgenic plants expressing a suitable member of the B. thuringiensis cry gene family could be a useful tool for overcoming this alfalfa yield constraint. The aim of this work was to produce alfalfa transgenic plants expressing a Bt protein and to assess its biological activity against C. lesbia under laboratory conditions.
Included in
Agricultural Science Commons, Agronomy and Crop Sciences Commons, Plant Biology Commons, Plant Pathology Commons, Soil Science Commons, Weed Science Commons
Development of Alfalfa (Medicago Sativa L.) Transgenic Plants Expressing a Bacillus Thuringiensis Endotoxin and Their Evaluation Against Alfalfa Caterpillar (Colias Lesbia)
Alfalfa (Medicago sativa L.) is the most important forage crop in Argentina, with ca. 6 million cultivated hectares. The production of this crop is limited by the alfalfa caterpillar (Colias lesbia) which causes a loss equivalent to at least 10% of the biomass per year. No natural tolerance against this lepidoptera was found in alfalfa germplasm, hampering the development of tolerant cultivars by conventional breeding. This pest is usually controlled by using chemical insecticides but this has adverse effects on beneficial insects and the environment. Alternatively, low doses of commercial Bt insecticides (40 to 70 g/ha) also proved to efficiently limit the pest. This observation leads to us consider that the development of alfalfa transgenic plants expressing a suitable member of the B. thuringiensis cry gene family could be a useful tool for overcoming this alfalfa yield constraint. The aim of this work was to produce alfalfa transgenic plants expressing a Bt protein and to assess its biological activity against C. lesbia under laboratory conditions.
