Satellite Symposium 5: Molecular Breeding
Description
Perennial ryegrass (Lolium perenne L.) and its fungal endophyte (Neotyphodium lolii) are known to establish a mutualistic association that impacts on the agronomic productivity of endophyte-infected ryegrass pastures. To study this interaction at the molecular level, a genomic resource consisting of 13,964 endophyte ESTs has been generated. However, the functions of a large proportion of these genes remain to be elucidated. Recent work has demonstrated the potential for RNA-mediated gene silencing to suppress gene expression in a sequence specific manner thus allowing for the subsequent analysis of gene function.
Citation
Felitti, S.; Tian, P.; Edwards, D.; and Spangenberg, G. C., "A High-Throughput Gene Silencing Approach for Studying the Interaction Between Perennial Ryegrass (Lolium Perenne) and the Fungal Endophyte Neotyphodium Lolii" (2023). IGC Proceedings (1993-2023). 62.
https://uknowledge.uky.edu/igc/20/satellitesymposium5/62
Included in
Agricultural Science Commons, Agronomy and Crop Sciences Commons, Plant Biology Commons, Plant Pathology Commons, Soil Science Commons, Weed Science Commons
A High-Throughput Gene Silencing Approach for Studying the Interaction Between Perennial Ryegrass (Lolium Perenne) and the Fungal Endophyte Neotyphodium Lolii
Perennial ryegrass (Lolium perenne L.) and its fungal endophyte (Neotyphodium lolii) are known to establish a mutualistic association that impacts on the agronomic productivity of endophyte-infected ryegrass pastures. To study this interaction at the molecular level, a genomic resource consisting of 13,964 endophyte ESTs has been generated. However, the functions of a large proportion of these genes remain to be elucidated. Recent work has demonstrated the potential for RNA-mediated gene silencing to suppress gene expression in a sequence specific manner thus allowing for the subsequent analysis of gene function.
