Satellite Symposium 5: Molecular Breeding
Description
A high-density linkage map of red clover was constructed based on SSR and RFLP markers. In order to construct a linkage map with user (breeder) friendly markers; i.e. informative and easy detection, two policies were adopted for marker development. One was that the markers should be derived from cDNA or gene-rich regions, and the other was that the SSR markers should be detected polymorphisms on agarose gels. We also discuss the transferability of the markers on the map to other red clover germplasm and legumes. Such highly transferable markers could be used to screen anchor markers for both on a consensus map of red clover and other legume species.
Citation
Isobe, S.; Sato, S.; Asamizu, E.; Klimenko, I.; Kozlov, N. N.; Okumura, K.; and Tabata, S., "A High-Density SSR Linkage Map of Red Clover and Its Transferability to Other Legumes" (2023). IGC Proceedings (1993-2023). 38.
https://uknowledge.uky.edu/igc/20/satellitesymposium5/38
Included in
Agricultural Science Commons, Agronomy and Crop Sciences Commons, Plant Biology Commons, Plant Pathology Commons, Soil Science Commons, Weed Science Commons
A High-Density SSR Linkage Map of Red Clover and Its Transferability to Other Legumes
A high-density linkage map of red clover was constructed based on SSR and RFLP markers. In order to construct a linkage map with user (breeder) friendly markers; i.e. informative and easy detection, two policies were adopted for marker development. One was that the markers should be derived from cDNA or gene-rich regions, and the other was that the SSR markers should be detected polymorphisms on agarose gels. We also discuss the transferability of the markers on the map to other red clover germplasm and legumes. Such highly transferable markers could be used to screen anchor markers for both on a consensus map of red clover and other legume species.
