Satellite Symposium 5: Molecular Breeding

Description

The temperate grass germplasm collection maintained at the USDA, ARS Western Regional Plant Introduction Station (WRPIS) in Pullman, Washington, consists of more than 18,000 accessions. Passport and collection data suggest that some of these accessions are duplicates, and their maintenance unnecessarily drains limited resources. The objective of this study was to use Amplified Fragment Length Polymorphism (AFLP) marker analysis on 4 populations of smooth bromegrass, Bromus inermis Leyss. subsp. inermis `Manchar ́, a cross-pollinated perennial grass, to determine if the genetic variation among them was significant. If not, then maintaining separate populations would be unnecessary.

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Analysis of Bromus Inermis Populations Using Amplified Fragment Length Polymorphism Markers to Identify Duplicate Accessions

The temperate grass germplasm collection maintained at the USDA, ARS Western Regional Plant Introduction Station (WRPIS) in Pullman, Washington, consists of more than 18,000 accessions. Passport and collection data suggest that some of these accessions are duplicates, and their maintenance unnecessarily drains limited resources. The objective of this study was to use Amplified Fragment Length Polymorphism (AFLP) marker analysis on 4 populations of smooth bromegrass, Bromus inermis Leyss. subsp. inermis `Manchar ́, a cross-pollinated perennial grass, to determine if the genetic variation among them was significant. If not, then maintaining separate populations would be unnecessary.