Publication Date
1993
Description
The objective of the present study was to assess the freezing tolerance using LT50 (temperature at which 50% mortality occurs) and to get information on changes of protein pattern in Italian ryegrass (Lolium multiflorum Lam.) after cold hardening. The correlation coefficient between LT50 and percentage of survival plants under field conditions was significantly high at 0.701 ". LT50 was considered to be useful to assess different genotypes and change of freezing tolerance during the winter. This technique will be used in breeding programmes to improve the freezing tolerance. Soluble proteins extracted from hardened and non-hardened seedlings were analysed by SOS-PAGE to clarify the relation to freezing tolerance. Quantitative changes in protein pattern and several new polypeptides were detected. These results indicated that the polypeptides may play an important role in the development of freezing tolerance.
Citation
Tase, K and Kobayashi, M, "Assessment of Freezing Tolerance and Changes of Protein Pattern in Italian Ryegrass after Cold Hardening" (2024). IGC Proceedings (1993-2023). 22.
https://uknowledge.uky.edu/igc/1993/session12/22
Included in
Agricultural Science Commons, Agronomy and Crop Sciences Commons, Plant Biology Commons, Plant Pathology Commons, Soil Science Commons, Weed Science Commons
Assessment of Freezing Tolerance and Changes of Protein Pattern in Italian Ryegrass after Cold Hardening
The objective of the present study was to assess the freezing tolerance using LT50 (temperature at which 50% mortality occurs) and to get information on changes of protein pattern in Italian ryegrass (Lolium multiflorum Lam.) after cold hardening. The correlation coefficient between LT50 and percentage of survival plants under field conditions was significantly high at 0.701 ". LT50 was considered to be useful to assess different genotypes and change of freezing tolerance during the winter. This technique will be used in breeding programmes to improve the freezing tolerance. Soluble proteins extracted from hardened and non-hardened seedlings were analysed by SOS-PAGE to clarify the relation to freezing tolerance. Quantitative changes in protein pattern and several new polypeptides were detected. These results indicated that the polypeptides may play an important role in the development of freezing tolerance.
