Publication Date
1993
Description
Horizontal starch gel electrophoresis of Chloris gayana (Rhodes grass) identified enzyme systems for use as selectable genetic markers and/or polymorphic loci for cultivllf differentiation, Most enzymes from crushed leaves of Rhodes grass showed anodal migration; but peroxidases exhibited Vllfiation in a multitude of anodal and cathodal bands. Glucose- 6-phosphate dehydrogenase and malate dehydrogenase had a single; locus-zone. Malic enzyme had one clear zone, but possibly another and a trace of cathodal activity, while phosphoglucose isomerase comprised two, designated PGl-1 and PGI-2, as did superoxide dismut115e (SOD-1 and SOD-2). Phosphoglucomutase had a clear fast and a fainter, slower zone-locus. 6-phosphogluconate dehydrogenase gave 2 zones (6PGDH- 1 and 6PGDH-2) as did leucine aminopeptidase. Esterase and acid phosphatase formed 2 or 3 regions, and glutamate oxaloacetate transaminase loci were designated GOT-I, GOT-2, and GOT-3. SOD-I gave a potentially valuable marker allele and PGI-2 was .the clearest polymorphic locus. Other systems of potential were esterase, acid phosphatase and glutamate oxaloacetate transaminase with polymorphic loci. Enzymes with single loci, and phosphoglucomutase, 6- phosphogluconate dehydrogenase, esterase, acid phosphatase may yield selectable genetic markers. Compatible enzymes for gel slicing are indicated.
Citation
York, P A., "Preliminary Isozyme Studies on Chloris gayana (Rhodes grass)" (2024). IGC Proceedings (1993-2023). 1.
https://uknowledge.uky.edu/igc/1993/session12/1
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Agricultural Science Commons, Agronomy and Crop Sciences Commons, Plant Biology Commons, Plant Pathology Commons, Soil Science Commons, Weed Science Commons
Preliminary Isozyme Studies on Chloris gayana (Rhodes grass)
Horizontal starch gel electrophoresis of Chloris gayana (Rhodes grass) identified enzyme systems for use as selectable genetic markers and/or polymorphic loci for cultivllf differentiation, Most enzymes from crushed leaves of Rhodes grass showed anodal migration; but peroxidases exhibited Vllfiation in a multitude of anodal and cathodal bands. Glucose- 6-phosphate dehydrogenase and malate dehydrogenase had a single; locus-zone. Malic enzyme had one clear zone, but possibly another and a trace of cathodal activity, while phosphoglucose isomerase comprised two, designated PGl-1 and PGI-2, as did superoxide dismut115e (SOD-1 and SOD-2). Phosphoglucomutase had a clear fast and a fainter, slower zone-locus. 6-phosphogluconate dehydrogenase gave 2 zones (6PGDH- 1 and 6PGDH-2) as did leucine aminopeptidase. Esterase and acid phosphatase formed 2 or 3 regions, and glutamate oxaloacetate transaminase loci were designated GOT-I, GOT-2, and GOT-3. SOD-I gave a potentially valuable marker allele and PGI-2 was .the clearest polymorphic locus. Other systems of potential were esterase, acid phosphatase and glutamate oxaloacetate transaminase with polymorphic loci. Enzymes with single loci, and phosphoglucomutase, 6- phosphogluconate dehydrogenase, esterase, acid phosphatase may yield selectable genetic markers. Compatible enzymes for gel slicing are indicated.
