Publication Date
1985
Location
Kyoto Japan
Description
Antiserum to purified Fraction 1 (18S) leaf protein (ribulose-1,5-bisphosphate carboxylase) raised in N .Z. white rabbits was used to analyse herbage, silage and digesta from the gastro-intestinal tract of sheep by crossed immuno-electrophoresis. Silage trials were carried out with luceme and ryegrass ensiled separately in mini silos containing 1 kg herbage and in larger silos containing 100 kg herbage with replications at 4 levels of dry matter, viz: direct cut, 18.8-21.6%; low wilt, 23.0-32.0%; medium wilt, 31.1-43.5% and high wilt, 38.5-53.2%. Under favourable weather conditions, with long hours of sunshine and low relative humidities and where the high wilt stage was reached within 24 h, there was no appreciable loss of immuno-reactive Fraction-1 protein during wilting of luceme or ryegrass. Under adverse conditions, with high relative humidity and reduced hours of sunshine, and where wilting required about 3 days luceme lost 71 % of its Fraction-1 protein. Ensiling was monitored by measurement of pH, VF A and lactate production and the rapidity and extent of fermentation was inversely proportional to the extent of wilting. In every luceme silage all Fraction-1 protein was degraded during fermentation. With ryegrass breakdown was different; in the mini silos large quantities (58-100%) of the Fraction-1 protein remained in the medium and high wilt silages whereas large losses occurred in the direct-cut and low wilt silages. In the larger scale trial each ryegrass silage showed the presence of 68-100% of the original Fraction-1 after fermentation. Digestibility studies in fistulated sheep showed that considerable amounts of the Fraction-1 protein in ryegrass silages reached the duodenum without breakdown.
Citation
Mangan, J L. and Harrison, F A., "Immuno-Reactive Fraction-1 (18S) Leaf Protein during Wilting and Ensiling of Lucerne (Medicago sativa L.) and Ryegrass (Lolium perenne L.)" (1985). IGC Proceedings (1985-2023). 5.
(URL: https://uknowledge.uky.edu/igc/1985/ses9/5)
Included in
Agricultural Science Commons, Agronomy and Crop Sciences Commons, Plant Biology Commons, Plant Pathology Commons, Soil Science Commons, Weed Science Commons
Immuno-Reactive Fraction-1 (18S) Leaf Protein during Wilting and Ensiling of Lucerne (Medicago sativa L.) and Ryegrass (Lolium perenne L.)
Kyoto Japan
Antiserum to purified Fraction 1 (18S) leaf protein (ribulose-1,5-bisphosphate carboxylase) raised in N .Z. white rabbits was used to analyse herbage, silage and digesta from the gastro-intestinal tract of sheep by crossed immuno-electrophoresis. Silage trials were carried out with luceme and ryegrass ensiled separately in mini silos containing 1 kg herbage and in larger silos containing 100 kg herbage with replications at 4 levels of dry matter, viz: direct cut, 18.8-21.6%; low wilt, 23.0-32.0%; medium wilt, 31.1-43.5% and high wilt, 38.5-53.2%. Under favourable weather conditions, with long hours of sunshine and low relative humidities and where the high wilt stage was reached within 24 h, there was no appreciable loss of immuno-reactive Fraction-1 protein during wilting of luceme or ryegrass. Under adverse conditions, with high relative humidity and reduced hours of sunshine, and where wilting required about 3 days luceme lost 71 % of its Fraction-1 protein. Ensiling was monitored by measurement of pH, VF A and lactate production and the rapidity and extent of fermentation was inversely proportional to the extent of wilting. In every luceme silage all Fraction-1 protein was degraded during fermentation. With ryegrass breakdown was different; in the mini silos large quantities (58-100%) of the Fraction-1 protein remained in the medium and high wilt silages whereas large losses occurred in the direct-cut and low wilt silages. In the larger scale trial each ryegrass silage showed the presence of 68-100% of the original Fraction-1 after fermentation. Digestibility studies in fistulated sheep showed that considerable amounts of the Fraction-1 protein in ryegrass silages reached the duodenum without breakdown.
