Presenter Information

G Pahlow, FAL
E Zimmer, FAL

Publication Date

1985

Location

Kyoto Japan

Description

The objective of the experiment was to optimize the fermentation process and to improve the aerobic stability of silage by the use of a bacterial additive. Prewilted grass was ensiled with and without the inoculant in laboratory silos. These were perfused with different amounts of oxygen to simulate suboptimum 5 ensiling technology. The spray inoculation of a Lactobacillus culture with 10 colony forming units (cfu) per g of the crop enabled the

homofermentative starter to dominate the indigenous flora even in the air treated silages. The lactic acid production was markedly increased and pH-value, gas losses and protein decomposition were generally reduced in the inoculated forage. Any supply of oxygen stimulated the multiplication of yeasts, which are responsible for aerobic instability. The bacterial additive restricted the development of these organisms up to a critical level of daily air infusion in the range between 100 and 200 mg oxygen (Oz)lkg dry matter (DM). These silages remained stable after unloading for at least 2 days. In all silages without inoculant even the lowest oxygen infusion caused yeast counts up to 107/g silage DM and an immediate start of deterioration after exposure to air. The better stability of the inoculated silages with low oxygen supply is attributed to a specific energy requiring detoxification system of the yeasts against too high intracellular concentrations of organic acids. For maintenance this mechanism either demands fermentable sugars which are mostly exhausted in these silages or a sufficient amount of oxygen to assimilate the organic acids available.

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Effect of a Lactobacillus Inoculant on Fermentation and Aerobic Stability of Grass Silage

Kyoto Japan

The objective of the experiment was to optimize the fermentation process and to improve the aerobic stability of silage by the use of a bacterial additive. Prewilted grass was ensiled with and without the inoculant in laboratory silos. These were perfused with different amounts of oxygen to simulate suboptimum 5 ensiling technology. The spray inoculation of a Lactobacillus culture with 10 colony forming units (cfu) per g of the crop enabled the

homofermentative starter to dominate the indigenous flora even in the air treated silages. The lactic acid production was markedly increased and pH-value, gas losses and protein decomposition were generally reduced in the inoculated forage. Any supply of oxygen stimulated the multiplication of yeasts, which are responsible for aerobic instability. The bacterial additive restricted the development of these organisms up to a critical level of daily air infusion in the range between 100 and 200 mg oxygen (Oz)lkg dry matter (DM). These silages remained stable after unloading for at least 2 days. In all silages without inoculant even the lowest oxygen infusion caused yeast counts up to 107/g silage DM and an immediate start of deterioration after exposure to air. The better stability of the inoculated silages with low oxygen supply is attributed to a specific energy requiring detoxification system of the yeasts against too high intracellular concentrations of organic acids. For maintenance this mechanism either demands fermentable sugars which are mostly exhausted in these silages or a sufficient amount of oxygen to assimilate the organic acids available.