Publication Date

1985

Location

Kyoto Japan

Description

The choke,a systemic disease, is a serious obstacle in the breeding and seed production of timothy in Japan, since the disease often prevents plant from normal heading. In this study, our goal was to cure the infected plants by systemic fungicide treatment. Among 15 chemicals tested, Bitertanol (Baycor) was most effective against the causal fungus, Epichloe typhina, in vitro tests, being completely fungicidal at 10 ppm. Triforine (Saprol) was effective being partially fungicidal at 10 ppm and completely fungicidal at 100 ppm. Benomyl, captafol+captan, chlorothalonil and triadimenol were fungistatic at 100 ppm and fungicidal at 1,000 ppm. In greenhouse test, only triforine displayed an excellent therapeutic activity on the infected seedlings, either by dipping of the seedlings to a 0.4% aqueous suspension for 24 hours or by application of 0.2% aqueous suspension( 8 liter/m2) to soil twice at 4-week intervals. The serial isolation tests revealed that the endophytic mycelia were eliminated after 8 weeks of treatment. In spite of its remarkable fungicidal activity in in vitro test, bitertanol showed no the therapeutic effect. The therapeutic activity of triforine was also confirmed in a heavily infested field, that is, the incidence of the disease was markedly suppressed by successive application to the soil on Apr. 26, Apr. 30 and May 18, 1982.

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Antifungal Activity of Systemic Fungicides on Mycelial Growth of Epichloe Typhina and Their Therapeutic Effects on the Choke Disease of Timothy

Kyoto Japan

The choke,a systemic disease, is a serious obstacle in the breeding and seed production of timothy in Japan, since the disease often prevents plant from normal heading. In this study, our goal was to cure the infected plants by systemic fungicide treatment. Among 15 chemicals tested, Bitertanol (Baycor) was most effective against the causal fungus, Epichloe typhina, in vitro tests, being completely fungicidal at 10 ppm. Triforine (Saprol) was effective being partially fungicidal at 10 ppm and completely fungicidal at 100 ppm. Benomyl, captafol+captan, chlorothalonil and triadimenol were fungistatic at 100 ppm and fungicidal at 1,000 ppm. In greenhouse test, only triforine displayed an excellent therapeutic activity on the infected seedlings, either by dipping of the seedlings to a 0.4% aqueous suspension for 24 hours or by application of 0.2% aqueous suspension( 8 liter/m2) to soil twice at 4-week intervals. The serial isolation tests revealed that the endophytic mycelia were eliminated after 8 weeks of treatment. In spite of its remarkable fungicidal activity in in vitro test, bitertanol showed no the therapeutic effect. The therapeutic activity of triforine was also confirmed in a heavily infested field, that is, the incidence of the disease was markedly suppressed by successive application to the soil on Apr. 26, Apr. 30 and May 18, 1982.