Publication Date
1985
Location
Kyoto Japan
Description
Effects of 2,4-D and kinetin on the growth of cells derived from alfalfa and their capacity to induce cold acclimation were investigated for the breeding of the cold hardy alfalfa using suspension culture. The yield was the highest in the modified Schnek and Hildebrandt medium containing 1 ppm 2,4-D and 0.5 ppm kinetin. Cells proliferated 12-fold in this medium at 26°C in the dark in ten days. Cells ceased growing during the cold acclimation and the pH in the medium kept unchanged. The cold hardiness of cells incubated at 1 °C in the dark increased during the first 20 days and thereafter decreased. The level of cold hardiness returned to the initial level 30 days after cold treatment. Cold hardiness reached maximum after 15 to 20 days. Dry matter percentages and nonreducing sugar contents changed in perallel with the cold hardiness during the period tested. Reducing sugar and starch were less in their contents than nonreducing sugar, being almost constant during the cold acclimation. The development of clod hardiness was related to the loss of water and to the accumulation of sugars. Present study revealed that cell suspension culture could be used for testing the cold hardiness of alfalfa.
Citation
Shibata, S, "Cold Acclimation of Alfalfa Cells in Suspension Culture" (1985). IGC Proceedings (1985-2023). 40.
(URL: https://uknowledge.uky.edu/igc/1985/ses3/40)
Included in
Agricultural Science Commons, Agronomy and Crop Sciences Commons, Plant Biology Commons, Plant Pathology Commons, Soil Science Commons, Weed Science Commons
Cold Acclimation of Alfalfa Cells in Suspension Culture
Kyoto Japan
Effects of 2,4-D and kinetin on the growth of cells derived from alfalfa and their capacity to induce cold acclimation were investigated for the breeding of the cold hardy alfalfa using suspension culture. The yield was the highest in the modified Schnek and Hildebrandt medium containing 1 ppm 2,4-D and 0.5 ppm kinetin. Cells proliferated 12-fold in this medium at 26°C in the dark in ten days. Cells ceased growing during the cold acclimation and the pH in the medium kept unchanged. The cold hardiness of cells incubated at 1 °C in the dark increased during the first 20 days and thereafter decreased. The level of cold hardiness returned to the initial level 30 days after cold treatment. Cold hardiness reached maximum after 15 to 20 days. Dry matter percentages and nonreducing sugar contents changed in perallel with the cold hardiness during the period tested. Reducing sugar and starch were less in their contents than nonreducing sugar, being almost constant during the cold acclimation. The development of clod hardiness was related to the loss of water and to the accumulation of sugars. Present study revealed that cell suspension culture could be used for testing the cold hardiness of alfalfa.
