Improved Efficiency of Plant Regeneration from Smooth Bromegrass (Bromus inermis) Suspension Culture
Publication Date
1985
Location
Kyoto Japan
Description
Smooth bromegrass, Bromus inermis Leyss, is the most important forage grass in Canada. Breeding for improvements such as higher yield and faster regrowth has made little progress, mainly due to the polyploidy and cross-pollination which are characteristic of this grass. The application of tissue culture methods to bromegrass improvement holds promise only if an efficient procedure for plant regeneration is found. Various basal culture media and auxins were tested for their effects on callus induction, growth of suspension cultures and plant regeneration. None of the tested auxins gave better in vitro growth than 2,4-D. Eriksson's medium with 2,4-D as the sole plant growth regulator was superior to all other tested media in suspension culture growth rate. The highest frequency of plant regeneration was obtained using the following regime:
1) growing suspension culture in Eriksson's medium supplemented with 2.5 µ.m 2,4-D, and
2) plating cell aggregates on hormone-free Kao's B medium containing 8 g/1 agar. Addition of 50 mM proline or glutamine to any of the regeneration media was inhibitory to plant regeneration.
Citation
Chen, T H. and Marowitch, J, "Improved Efficiency of Plant Regeneration from Smooth Bromegrass (Bromus inermis) Suspension Culture" (1985). IGC Proceedings (1985-2023). 27.
(URL: https://uknowledge.uky.edu/igc/1985/ses3/27)
Included in
Agricultural Science Commons, Agronomy and Crop Sciences Commons, Plant Biology Commons, Plant Pathology Commons, Soil Science Commons, Weed Science Commons
Improved Efficiency of Plant Regeneration from Smooth Bromegrass (Bromus inermis) Suspension Culture
Kyoto Japan
Smooth bromegrass, Bromus inermis Leyss, is the most important forage grass in Canada. Breeding for improvements such as higher yield and faster regrowth has made little progress, mainly due to the polyploidy and cross-pollination which are characteristic of this grass. The application of tissue culture methods to bromegrass improvement holds promise only if an efficient procedure for plant regeneration is found. Various basal culture media and auxins were tested for their effects on callus induction, growth of suspension cultures and plant regeneration. None of the tested auxins gave better in vitro growth than 2,4-D. Eriksson's medium with 2,4-D as the sole plant growth regulator was superior to all other tested media in suspension culture growth rate. The highest frequency of plant regeneration was obtained using the following regime:
1) growing suspension culture in Eriksson's medium supplemented with 2.5 µ.m 2,4-D, and
2) plating cell aggregates on hormone-free Kao's B medium containing 8 g/1 agar. Addition of 50 mM proline or glutamine to any of the regeneration media was inhibitory to plant regeneration.
