Publication Date
1985
Location
Kyoto Japan
Description
The mechanism of phlein degradation in the stem bases following defoliation was studied using timothy, orchardgrass, tall fescue and Italian ryegrass. The phleinase(phlein hydrolyzing enzyme) was induced in the stem bases of all these grasses after defoliation, and the variation patterns of the activity varied with the grass species. The activity was inversely related to the phlein concentration, suggesting that the phleinase is involved in the degradation of phlein in all these grasses. The variation pattern of phleinase in orchardgrass was almost the same under different growth conditions, while that was amplified with high temperature, long day condition or N fertilization. The phleinase from these grasses attacks a terminal fructosyl residue of phlein. The velocity of fructose release from phlein by the phleinase from timothy and orchardgrass was proportional to the molarity of phlein irrespective of its chain length. The phleinase from orchardgrass cleaved phlein(��-2,6-fructan), triticin {branched ��-2,6-fructan with ��-2,1 linkage) and inulin(��-2,1-fructan) in order of affinity, but not sucrose.
Citation
Yamamoto, Shinro and Mino, Yosuke, "Phlein Degradation in Stem Bases of Temperature Grasses After Defoliation" (1985). IGC Proceedings (1985-2023). 14.
(URL: https://uknowledge.uky.edu/igc/1985/ses3/14)
Included in
Agricultural Science Commons, Agronomy and Crop Sciences Commons, Plant Biology Commons, Plant Pathology Commons, Soil Science Commons, Weed Science Commons
Phlein Degradation in Stem Bases of Temperature Grasses After Defoliation
Kyoto Japan
The mechanism of phlein degradation in the stem bases following defoliation was studied using timothy, orchardgrass, tall fescue and Italian ryegrass. The phleinase(phlein hydrolyzing enzyme) was induced in the stem bases of all these grasses after defoliation, and the variation patterns of the activity varied with the grass species. The activity was inversely related to the phlein concentration, suggesting that the phleinase is involved in the degradation of phlein in all these grasses. The variation pattern of phleinase in orchardgrass was almost the same under different growth conditions, while that was amplified with high temperature, long day condition or N fertilization. The phleinase from these grasses attacks a terminal fructosyl residue of phlein. The velocity of fructose release from phlein by the phleinase from timothy and orchardgrass was proportional to the molarity of phlein irrespective of its chain length. The phleinase from orchardgrass cleaved phlein(��-2,6-fructan), triticin {branched ��-2,6-fructan with ��-2,1 linkage) and inulin(��-2,1-fructan) in order of affinity, but not sucrose.
