Publication Date
1985
Location
Kyoto Japan
Description
Young inflorescences of pangola grass, (Digitaria decumbens Stent), CV. A24, were cultured on half strength of Murashige and Skoog's (MS) medium supplemented with various concentrations and combinations of2,4-dichlorophenoxyacetic acid (2,4-D), naphthaleneacetic acid (NAA) and kinetin to find the optimum medium for callus induction and plant regeneration. Callus tissue was initiated from the intercalary meristems of the spikelet base or rachis of the cultured inflorescences on either 2,4-D or NAA medium. Calluses were induced 6 days after culturing in 4mg/1 2,4-D and 2mg/1 kinetin medium. Plantlets were obtained in 5 days after calluses were transplanted on MS medium not supplemented with 2,4-D. In medium with 4mg/1 NAA and 2mg/1 kinetin, callus was induced in 7 days and plantlets were regenerated in 14 days after culturing. The plantlets were regenerated via callus or somatic embryogenesis. Younger inflorescences produced more callus than the older ones. The use of whole inflorescences as a source of explants would not only provide ample areas of intercalary meristematic tissues for rapid initiation of callus, but also simplify the procedures of disinfecting the inocula. Plant regenerated from young inflorescence cultures was shown to have normal chromosome number of 2n=18. However, abnormal pollens were observed in mature plants.
Citation
Cheng, Y K., "Callus Induction and Plant Regeneration from Inflorescence Segments of Pangola Grass" (1985). IGC Proceedings (1985-2023). 48.
(URL: https://uknowledge.uky.edu/igc/1985/ses2/48)
Included in
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Callus Induction and Plant Regeneration from Inflorescence Segments of Pangola Grass
Kyoto Japan
Young inflorescences of pangola grass, (Digitaria decumbens Stent), CV. A24, were cultured on half strength of Murashige and Skoog's (MS) medium supplemented with various concentrations and combinations of2,4-dichlorophenoxyacetic acid (2,4-D), naphthaleneacetic acid (NAA) and kinetin to find the optimum medium for callus induction and plant regeneration. Callus tissue was initiated from the intercalary meristems of the spikelet base or rachis of the cultured inflorescences on either 2,4-D or NAA medium. Calluses were induced 6 days after culturing in 4mg/1 2,4-D and 2mg/1 kinetin medium. Plantlets were obtained in 5 days after calluses were transplanted on MS medium not supplemented with 2,4-D. In medium with 4mg/1 NAA and 2mg/1 kinetin, callus was induced in 7 days and plantlets were regenerated in 14 days after culturing. The plantlets were regenerated via callus or somatic embryogenesis. Younger inflorescences produced more callus than the older ones. The use of whole inflorescences as a source of explants would not only provide ample areas of intercalary meristematic tissues for rapid initiation of callus, but also simplify the procedures of disinfecting the inocula. Plant regenerated from young inflorescence cultures was shown to have normal chromosome number of 2n=18. However, abnormal pollens were observed in mature plants.
