Publication Date
1985
Location
Kyoto Japan
Description
A method was proposed for selection of mutants in cross-fertilizing plant species. It is composed of taking seeds separately from each spike of the mutagen-treated population, sowing the seeds in hill plots in the next generation, isolating each hill from the others by bagging all the plants of each hill at flowering time, taking seeds for the next generation from each hill, and sowing them in hill progenies for selection of mutants. The procedure will be called "Crossing-within-Spike-Progeny" method, or simply CSP method. To increase the frequency of mutant genes and to enlarge the mutated sector within spike in the population to which the CSP method is to be applied make the method more effective. Using populations of Italian ryegrass treated with gamma-rays and a chemical mutagen, the efficiency of the CSP method was tested. After six cycles of 30 kR of gamma-ray exposures chlorophyll mutation frequency reached as high as 70% per hill progeny and 1.87 % per plant when CSP method was applied, while the same population produced a frequency of only 10.0 % per progeny and 0.12 % per plant when open-pollinated. Thus, CSP method was found to be highly effective for increasing the chance of obtaining mutants in cross-fertilizing plant species.
Citation
Ukai, Y, "A New Method for Selection of Mutants in Cross-Fertilizing Species" (1985). IGC Proceedings (1985-2023). 37.
(URL: https://uknowledge.uky.edu/igc/1985/ses2/37)
Included in
Agricultural Science Commons, Agronomy and Crop Sciences Commons, Plant Biology Commons, Plant Pathology Commons, Soil Science Commons, Weed Science Commons
A New Method for Selection of Mutants in Cross-Fertilizing Species
Kyoto Japan
A method was proposed for selection of mutants in cross-fertilizing plant species. It is composed of taking seeds separately from each spike of the mutagen-treated population, sowing the seeds in hill plots in the next generation, isolating each hill from the others by bagging all the plants of each hill at flowering time, taking seeds for the next generation from each hill, and sowing them in hill progenies for selection of mutants. The procedure will be called "Crossing-within-Spike-Progeny" method, or simply CSP method. To increase the frequency of mutant genes and to enlarge the mutated sector within spike in the population to which the CSP method is to be applied make the method more effective. Using populations of Italian ryegrass treated with gamma-rays and a chemical mutagen, the efficiency of the CSP method was tested. After six cycles of 30 kR of gamma-ray exposures chlorophyll mutation frequency reached as high as 70% per hill progeny and 1.87 % per plant when CSP method was applied, while the same population produced a frequency of only 10.0 % per progeny and 0.12 % per plant when open-pollinated. Thus, CSP method was found to be highly effective for increasing the chance of obtaining mutants in cross-fertilizing plant species.
