Publication Date
1985
Location
Kyoto Japan
Description
First cytoplasmic male sterility ( abbreviated as CMS) was induced by chemical mutagens. By treating dried seeds of type-0 strains with an aqueous solution of acriflavine, streptomycin, acridine orange and ethidium bromide, at various concentrations and by raising M1 plants from the above seeds, the authorswere able to find certain male sterile variants (S.S.b and C.S. type) in the M1 lines. Moreover, this male sterility was transmitted from the M1 plants to their M2 lines at relatively high frequencies, i.e., over 50%. It was demonstrated that the normal cytoplasm converted to the sterile cytoplasm which is equivalent with S cytoplasm from spontaneous origin. Conversely, dried seeds from CMS strains, TK76-MS and TK81-MS were treated with the aqueous solution (1000 ppm or 2000 ppm) of streptomycin for 24 hrs. In M1 generation, male fertile rev��rtants showing N type were induced in the treatment with streptomycin in TK76-MS, while no fertile plants appeared in the non-treated plots. In M2 generation, male fertility (Nor S.S.a type) was bred true in 40 progenies, while only one male sterile (S.S.b or C.S.) plant was segregated in the other four progenies. From the experimental results it was plausible that the reverse mutation from S to N cytoplasm was induced by the treatment. The artificial induction of male sterile cytoplasm may be a very useful tool for hybrid seed production of fodder and sugar beets.
Citation
Kinoshita, T, "Induction of Cytoplasmic Mutation on Male Sterility in Beta vulgaris L." (1985). IGC Proceedings (1985-2023). 32.
(URL: https://uknowledge.uky.edu/igc/1985/ses2/32)
Included in
Agricultural Science Commons, Agronomy and Crop Sciences Commons, Plant Biology Commons, Plant Pathology Commons, Soil Science Commons, Weed Science Commons
Induction of Cytoplasmic Mutation on Male Sterility in Beta vulgaris L.
Kyoto Japan
First cytoplasmic male sterility ( abbreviated as CMS) was induced by chemical mutagens. By treating dried seeds of type-0 strains with an aqueous solution of acriflavine, streptomycin, acridine orange and ethidium bromide, at various concentrations and by raising M1 plants from the above seeds, the authorswere able to find certain male sterile variants (S.S.b and C.S. type) in the M1 lines. Moreover, this male sterility was transmitted from the M1 plants to their M2 lines at relatively high frequencies, i.e., over 50%. It was demonstrated that the normal cytoplasm converted to the sterile cytoplasm which is equivalent with S cytoplasm from spontaneous origin. Conversely, dried seeds from CMS strains, TK76-MS and TK81-MS were treated with the aqueous solution (1000 ppm or 2000 ppm) of streptomycin for 24 hrs. In M1 generation, male fertile rev��rtants showing N type were induced in the treatment with streptomycin in TK76-MS, while no fertile plants appeared in the non-treated plots. In M2 generation, male fertility (Nor S.S.a type) was bred true in 40 progenies, while only one male sterile (S.S.b or C.S.) plant was segregated in the other four progenies. From the experimental results it was plausible that the reverse mutation from S to N cytoplasm was induced by the treatment. The artificial induction of male sterile cytoplasm may be a very useful tool for hybrid seed production of fodder and sugar beets.
