Publication Date
1985
Location
Kyoto Japan
Description
A program was designed to obtain variation of bahiagrass (Paspalum notatum Fliigge) and rhodesgrass ( Chloris gayana Kunth) by tissue culture. Callus was obtained from seeds of bahiagrass and rhodesgrass or panicles of rhodesgrass on callus formation mediums. Regeneration of complete plants was accomplished by transferring callus to regeneration mediums. Variation of regenerated plants was observed in such characters as plant type, leaf width, anthocyanin coloration, grain length etc. One variant of bahiagrass showed wider leaves, darker green leaves and larger grains than the original and tetraploid (2n =40). One variant of rhodesgrass showed wider leaves, thicker stems and larger racemes than the original but was diploid (2n=20). These two plants were observed to be useful for breeding materials. This program has led to being possibility of the use of tissue culture techniques in bahiagrass and rhodesgrass breeding programs.
Citation
Doi, O; Tsurumi, Y; and Nagatani, T, "Variation in Plants Regenerated from Tissue Culture of Bahiagrass and Rhodesgrass" (1985). IGC Proceedings (1985-2023). 30.
(URL: https://uknowledge.uky.edu/igc/1985/ses2/30)
Included in
Agricultural Science Commons, Agronomy and Crop Sciences Commons, Plant Biology Commons, Plant Pathology Commons, Soil Science Commons, Weed Science Commons
Variation in Plants Regenerated from Tissue Culture of Bahiagrass and Rhodesgrass
Kyoto Japan
A program was designed to obtain variation of bahiagrass (Paspalum notatum Fliigge) and rhodesgrass ( Chloris gayana Kunth) by tissue culture. Callus was obtained from seeds of bahiagrass and rhodesgrass or panicles of rhodesgrass on callus formation mediums. Regeneration of complete plants was accomplished by transferring callus to regeneration mediums. Variation of regenerated plants was observed in such characters as plant type, leaf width, anthocyanin coloration, grain length etc. One variant of bahiagrass showed wider leaves, darker green leaves and larger grains than the original and tetraploid (2n =40). One variant of rhodesgrass showed wider leaves, thicker stems and larger racemes than the original but was diploid (2n=20). These two plants were observed to be useful for breeding materials. This program has led to being possibility of the use of tissue culture techniques in bahiagrass and rhodesgrass breeding programs.
