Theme 12: Forage Breeding and Genetics
Description
Rhodes grass (Chloris gayana Kunth), a C4 forage grass, is regarded as salttolerant and exhibits intra- and inter-cultivar variability for this trait. Plants of cv Boma were selected for salt tolerance at the seedling and adult stages, cloned and characterized by RAPD and AFLP amplification patterns. Both techniques were equally efficient for fingerprinting these clones. More bands were obtained by AFLP but the ratio of polymorphic bands and the proportion present only in tolerant clones were the same by both methods. These bands, along with those exclusive for sensitive clones could be useful as markers for assisted selection.
Included in
Searching for Molecular Markers for Salt Tolerance in Rhodes Grass (Chloris gayana Kunth)
Rhodes grass (Chloris gayana Kunth), a C4 forage grass, is regarded as salttolerant and exhibits intra- and inter-cultivar variability for this trait. Plants of cv Boma were selected for salt tolerance at the seedling and adult stages, cloned and characterized by RAPD and AFLP amplification patterns. Both techniques were equally efficient for fingerprinting these clones. More bands were obtained by AFLP but the ratio of polymorphic bands and the proportion present only in tolerant clones were the same by both methods. These bands, along with those exclusive for sensitive clones could be useful as markers for assisted selection.
