BACKGROUND: The recent advancements in high-throughput sequencing have resulted in the availability of annotated genomes, as well as of multi-omics data for many living organisms. This has increased the need for graphic tools that allow the concurrent visualization of genomes and feature-associated multi-omics data on single publication-ready plots.
RESULTS: We present chromoMap, an R package, developed for the construction of interactive visualizations of chromosomes/chromosomal regions, mapping of any chromosomal feature with known coordinates (i.e., protein coding genes, transposable elements, non-coding RNAs, microsatellites, etc.), and chromosomal regional characteristics (i.e. genomic feature density, gene expression, DNA methylation, chromatin modifications, etc.) of organisms with a genome assembly. ChromoMap can also integrate multi-omics data (genomics, transcriptomics and epigenomics) in relation to their occurrence across chromosomes. ChromoMap takes tab-delimited files (BED like) or alternatively R objects to specify the genomic co-ordinates of the chromosomes and elements to annotate. Rendered chromosomes are composed of continuous windows of a given range, which, on hover, display detailed information about the elements annotated within that range. By adjusting parameters of a single function, users can generate a variety of plots that can either be saved as static image or as HTML documents.
CONCLUSIONS: ChromoMap's flexibility allows for concurrent visualization of genomic data in each strand of a given chromosome, or of more than one homologous chromosome; allowing the comparison of multi-omic data between genotypes (e.g. species, varieties, etc.) or between homologous chromosomes of phased diploid/polyploid genomes. chromoMap is an extensive tool that can be potentially used in various bioinformatics analysis pipelines for genomic visualization of multi-omics data.
Digital Object Identifier (DOI)
CMRL is partially supported by the National Institute of Food and Agriculture, AFRI Competitive Grant Program Accession No. 1018617 and National Institute of Food and Agriculture, United States Department of Agriculture, Hatch Program Accession No. 1020852.
All datasets used to showcase chromoMap functionalities were previously published and are cited in the text. Datasets used to generate Figs. 1, 4b and 5 were obtained from the NCBI Gene Expression Omnibus, IDs: GSE162835 GSE59055, GSE42081 respectively. Data used for Fig. 4a was obtained from the European Nucleotide Archive: Study Accession No. PRJEB27251. Data used for Figs. 2 and 3 was obtained from Ensembl BioMart.
Anand, Lakshay and Rodriguez Lopez, Carlos M., "ChromoMap: An R Package for Interactive Visualization of Multi-Omics Data and Annotation of Chromosomes" (2022). Horticulture Faculty Publications. 59.