Role of Satellite Cells in Long Term Fiber Type Shifts

Author

Honglu Liu, University of KentuckyFollow

Year of Publication

2013

College

Education

Department/School/Program

Rehabilitation Sciences

Degree Name

Bachelor of Science in Education

First Capstone/Thesis Advisor

Dr. Charlotte Peterson

Abstract

Muscle fiber type shifts in respect to satellite cells, muscle stem cells, are not well understood currently. The Peterson Lab has generated a mouse model (PAX7-DTA) that ablates satellite cells to determine if these muscle stem cells contribute to mouse muscle fiber type changes over an eight week period. In the study, control and satellite-cell-ablated mouse groups were split into control and overload groups (via synergist ablation surgery) and placed under similar environmental conditions. Eight weeks post-experiment, muscles were dissected to obtain the plantaris muscles of animals from all groups. Muscle cross-sections obtained were immunohistochemically stained, imaged, quantified by fiber type, and statistically analyzed. Results of the study found that in a two-way ANOVA analysis, there was no significant difference (P > 0.05) between groups. This result suggests that satellite cells play little apparent role in muscle fiber type shifts in mouse muscle in response to overload.

Recommended Citation

Liu, Honglu, "Role of Satellite Cells in Long Term Fiber Type Shifts" (2013). Lewis Honors College Capstone Collection. 4.
https://uknowledge.uky.edu/honprog/4