1. Drug administration studies using diisopropylamine dichloroacetate (DADA) and diisopropylamine (DIPA) were conducted in Thoroughbred and Standardbred horses to assess physiological effects and develop detection methods.
2. Four horses received 0.08 mg DADA/kg body wt and showed no changes in heart and respiratory rates or body temperature as measured over a 1-hr period after administration. A transient diuretic effect was found to occur in 2 mares dosed with 0.80 mg DADA/kg body wt.
3. A qualitative detection method using thin-layer chromatography was developed to detect DIPA, the major metabolite of DADA in equine urine. A quantitative detection method (lower limit of detection 0.5/zg/ml urine) for this metabolite was also developed using gas chromatography.
4. Neither DADA or the free base, DIPA, were detectable in equine blood samples using the abovementioned methodologies.
Supported by a grant entitled "Immunoassay Tests for High Potency Narcotic Analgesics in Racing Horses" from the Kentucky Equine Drug Research Council and the Kentucky State Racing and Harness Racing Commissions.
Yang, J.-M.; Woods, W. E.; Weckman, T. J.; Wood, T. W.; Chang, S.-L.; Blake, J. W.; and Tobin, Thomas, "THE DETECTION, PHARMACOKINETICS AND BEHAVIORAL EFFECTS OF DIISOPROPYLAMINE DICHLOROACETATE (DADA) IN THE HORSE: A PRELIMINARY REPORT" (1988). Maxwell H. Gluck Equine Research Center Faculty Publications. 89.