Immunization with an insect cell lysate/baculovirus mixture containing recombinant porcine epidemic diarrhea virus (PEDV) spike protein induced high levels of neutralizing antibodies in both mice and piglets. However, immunization of piglets with this vaccine resulted in enhancement of disease symptoms and virus replication in vaccine recipients exposed to PEDV challenge. Thus, these observations demonstrate a previously unrecognized challenge of PEDV vaccine research, which has important implications for coronavirus vaccine development.
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The work was supported by USDA/NIFA 2016-67016-24949, Kentucky Agricultural Experiment Station, and William Robert Mills Endowment Fund.
The raw data used for the preparation of figures of this study are available from the corresponding author upon reasonable request.
Yu, Jieshi; Sreenivasan, Chithra; Uprety, Tirth; Gao, Rongyuan; Huang, Chen; Lee, Ella J.; Lawson, Steven; Nelson, Julie; Christopher-Hennings, Jane; Kaushik, Radhey S.; Nelson, Eric; Diel, Diego G.; Hause, Ben M.; Li, Feng; and Wang, Dan, "Piglet Immunization with a Spike Subunit Vaccine Enhances Disease by Porcine Epidemic Diarrhea Virus" (2021). Maxwell H. Gluck Equine Research Center Faculty Publications. 51.