Abstract
EcoHIV is a model of HIV infection that recapitulates aspects of HIV-1 pathology in mice. However, there are limited published protocols to guide EcoHIV virion production. Here, we present a protocol for producing infective EcoHIV virions and essential quality controls. We describe steps for viral purification, titering, and multiple techniques to analyze infection efficacy. This protocol produces high infectivity in C57BL/6 mice which will aid investigators in generating preclinical data.
Document Type
Article
Publication Date
9-2023
Digital Object Identifier (DOI)
https://doi.org/10.1016/j.xpro.2023.102368
Funding Information
This work is supported by grants from the Na- tional Institutes of Health, National Heart, Lung, and Blood Institute (HL56652, HL138179, and HL150818 to S.W.W.).
Repository Citation
Alfar, Hammodah Rawhi Hammodah; Pariser, Daphne N.; Chanzu, Harry; Joshi, Smita; Coenen, Daniёlle M.; Lykins, Joshua; Prakhya, Kanakanagavalli Shravani; Potash, Mary Jane; Chao, Wei; Kelschenbach, Jennifer; Volsky, David J.; and Metcalf-Pate, Kelly, "Protocol for optimizing production and quality control of infective EcoHIV virions" (2023). Saha Cardiovascular Research Center Faculty Publications. 68.
https://uknowledge.uky.edu/cvrc_facpub/68
Included in
Biochemistry Commons, Cardiology Commons, Circulatory and Respiratory Physiology Commons, Genetics Commons, Molecular Biology Commons, Other Immunology and Infectious Disease Commons, Other Microbiology Commons, Other Neuroscience and Neurobiology Commons
Notes/Citation Information
© 2023 The Authors.