Serum Amyloid A Is Not Incorporated into HDL during HDL Biogenesis

Authors

Ailing Ji, University of KentuckyFollow
Xuebing Wang, University of Kentucky
Victoria P. Noffsinger, University of KentuckyFollow
Drew Jennings, University of KentuckyFollow
Maria C. de Beer, University of KentuckyFollow
Frederick C. de Beer, University of KentuckyFollow
Lisa R. Tannock, University of KentuckyFollow
Nancy R. Webb, University of KentuckyFollow

Abstract

Liver-derived serum amyloid A (SAA) is present in plasma where it is mainly associated with HDL and from which it is cleared more rapidly than are the other major HDL-associated apolipoproteins. Although evidence suggests that lipid-free and HDL-associated forms of SAA have different activities, the pathways by which SAA associates and disassociates with HDL are poorly understood. In this study, we investigated SAA lipidation by hepatocytes and how this lipidation relates to the formation of nascent HDL particles. We also examined hepatocyte-mediated clearance of lipid-free and HDL-associated SAA. We prepared hepatocytes from mice injected with lipopolysaccharide or an SAA-expressing adenoviral vector. Alternatively, we incubated primary hepatocytes from SAA-deficient mice with purified SAA. We analyzed conditioned media to determine the lipidation status of endogenously produced and exogenously added SAA. Examining the migration of lipidated species, we found that SAA is lipidated and forms nascent particles that are distinct from apoA-I-containing particles and that apoA-I lipidation is unaltered when SAA is overexpressed or added to the cells, indicating that SAA is not incorporated into apoA-I-containing HDL during HDL biogenesis. Like apoA-I formation, generation of SAA-containing particles was dependent on ABCA1, but not on scavenger receptor class B type I. Hepatocytes degraded significantly more SAA than apoA-I. Taken together, our results indicate that SAA's lipidation and metabolism by the liver is independent of apoA-I and that SAA is not incorporated into HDL during HDL biogenesis.

Document Type

Article

Publication Date

3-2020

Notes/Citation Information

Published in Journal of Lipid Research, v. 61, issue 3.

Copyright © 2020 Ji et al.

This is an Open Access article under the CC BY license.

Digital Object Identifier (DOI)

https://doi.org/10.1194/jlr.RA119000329

Funding Information

This work was supported by National Institutes of Health Grant R01 HL134731 (to N.R.W. and F.C.d.B.) and U.S. Department of Veterans Affairs Grants CX000773 (to N.R.W.), and CX000975 (to L.R.T.).

Repository Citation

Ji, Ailing; Wang, Xuebing; Noffsinger, Victoria P.; Jennings, Drew; de Beer, Maria C.; de Beer, Frederick C.; Tannock, Lisa R.; and Webb, Nancy R., "Serum Amyloid A Is Not Incorporated into HDL during HDL Biogenesis" (2020). Saha Cardiovascular Research Center Faculty Publications. 47.
https://uknowledge.uky.edu/cvrc_facpub/47