Abstract
We describe the ability of an engineered glycosyltransferase (OleD Loki) to catalyze the N‐glycosylation of tertiary‐amine‐containing drugs and trichostatin hydroxamate glycosyl ester formation. As such, this study highlights the first bacterial model catalyst for tertiary‐amine N‐glycosylation and further expands the substrate scope and synthetic potential of engineered OleDs. In addition, this work could open the door to the discovery of similar capabilities among other permissive bacterial glycosyltransferases.
Document Type
Article
Publication Date
2-16-2017
Digital Object Identifier (DOI)
https://doi.org/10.1002/cbic.201600676
Funding Information
This work was supported, in part, by National Institute of Health grants R37 AI52218 (J.S.T.) and U01 GM098248 (G.N.P.), the National Center for Advancing Translational Sciences (UL1TR001998), the University of Kentucky College of Pharmacy and the University of Kentucky Center for Pharmaceutical Research and Innovation.
Related Content
Supporting information for this article can be found under: https://doi.org/10.1002/cbic.201600676.
Repository Citation
Hughes, Ryan R.; Shaaban, Khaled A.; Zhang, Jianjun; Cao, Hongnan; Phillips, George N. Jr.; and Thorson, Jon S., "OleD Loki as a Catalyst for Tertiary Amine and Hydroxamate Glycosylation" (2017). Center for Pharmaceutical Research and Innovation Faculty Publications. 5.
https://uknowledge.uky.edu/cpri_facpub/5
Supporting Information
Included in
Biochemistry, Biophysics, and Structural Biology Commons, Pharmacy and Pharmaceutical Sciences Commons
Notes/Citation Information
Published in ChemBioChem, v. 18, issue 4, p. 363-367.
© 2017 Wiley‐VCH Verlag GmbH & Co. KGaA, Weinheim
The copyright holder has granted the permission for posting the article here.
This is the peer reviewed version of the following article: Hughes, R. R., Shaaban, K. A., Zhang, J., Cao, H., Phillips, G. N., Jr., & Thorson, J. S. (2017). OleD Loki as a catalyst for tertiary amine and hydroxamate glycosylation. ChemBioChem, 18(4), 363-367, which has been published in final form at https://doi.org/10.1002/cbic.201600676. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.