Archived
This content is available here strictly for research, reference, and/or recordkeeping and as such it may not be fully accessible. If you work or study at University of Kentucky and would like to request an accessible version, please use the SensusAccess Document Converter.
US Patent Number
7345160
Publication Date
3-18-2008
Abstract
The invention relates to aequorin and obelin mutants whose emission is shifted with respect to wild type. The shift in emission is accomplished using a combination of mutations of amino acids within aequorin or obelin that affect bioluminescence; use of different types of chromophores, i.e., coelenterazines with variable emission characteristics; and modifications of the photoprotein with fluorophores that will allow for emission of light at longer wavelengths as a result of energy transfer. Additionally, an assay employing aequorin mutants to test for HIV-1 protease inhibitors is disclosed.
Assignees
University of Kentucky Research Foundation, Lexington, KY (US)
Application Number
10/811,138
Filing Date
03/29/2004
Recommended Citation
Daunert, Sylvia; Deo, Sapna K.; Dikici, Emre; and Rowe, Laura, "Aequorin and Obelin Mutants with Differing Wavelengths and Bioluminescence" (2008). Chemistry Faculty Patents. 13.
https://uknowledge.uky.edu/chemistry_patents/13
