THE DIFFERENCES BETWEEN IRON AND IRON-SUBSTITUTED MANGANESE SUPEROXIDE DISMUTASE WITH RESPECT TO HYDROGEN PEROXIDE TREATMENT

Author

Jianing Wang, University of KentuckyFollow

Date Available

5-6-2014

Year of Publication

2014

Document Type

Master's Thesis

Degree Name

Master of Science (MS)

College

Arts and Sciences

Department/School/Program

Chemistry

Advisor

Dr. Jianing Wang

Abstract

Iron-substituted manganese superoxide dismutase (Fe(Mn)SOD) was produced using an in vivo preparation method. It’s an inactive enzyme in catalyzing superoxide radical dismutation owing to the mis-incorporation of Fe in the active site evolved to use Mn. To investigate the possible toxicity of human Fe(Mn)SOD proposed by Yamakura, we studied the properties of Fe(Mn)SOD upon H₂O₂ treatment and compared to that of FeSOD. It’s found that the responses to H₂O₂ treatment were different, including the changes of optical spectra, variations of active site coordination and secondary structures. Fe³⁺ reduction was not observed in Fe(Mn)SOD even H₂O₂ is believed to oxidize proteins via highly reactive intermediates including Fe and formed via Fe²⁺, which is true in FeSOD. What’s more, the activities of Fe(Mn)SOD and FeSOD were totally different in the ABTS assay or Amplex Red assay. These results indicated that the mechanism of peroxidase reaction of Fe(Mn)SOD is not identical to that of FeSOD.

Recommended Citation

Wang, Jianing, "THE DIFFERENCES BETWEEN IRON AND IRON-SUBSTITUTED MANGANESE SUPEROXIDE DISMUTASE WITH RESPECT TO HYDROGEN PEROXIDE TREATMENT" (2014). Theses and Dissertations--Chemistry. 37.
https://uknowledge.uky.edu/chemistry_etds/37