Metabolic reprogramming is a hallmark of cancer. The changes in metabolism are adaptive to permit proliferation, survival, and eventually metastasis in a harsh environment. Stable isotope-resolved metabolomics (SIRM) is an approach that uses advanced approaches of NMR and mass spectrometry to analyze the fate of individual atoms from stable isotope-enriched precursors to products to deduce metabolic pathways and networks. The approach can be applied to a wide range of biological systems, including human subjects. This review focuses on the applications of SIRM to cancer metabolism and its use in understanding drug actions.

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Published in The Journal of Biological Chemistry, v. 292, no. 28, p. 11601-11609.

This research was originally published in The Journal of Biological Chemistry. Ronald C. Bruntz, Andrew N. Lane, Richard M. Higashi, and Teresa W.-M. Fan. Exploring Cancer Metabolism Using Stable Isotope-Resolved Metabolomics (SIRM). J. Biol. Chem. 2017; 292:11601-11609. © 2017 by The American Society for Biochemistry and Molecular Biology, Inc.

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This work was supported in part by National Institutes of Health Grants T32CA165990 (to R. C. B.), 1R01ES022191-01 (to T. W. M. F. and R. M. H.), and 1P01CA163223-01A1 (to A. N. L. and T. W. M. F.).