Discovery of Sex-Specific Regions in a Salamander Genome

Presenter Information

Nataliya Y. Timoshevskaya, University of KentuckyFollow
Melissa C. Keinath, University of KentuckyFollow
Jeramiah J. Smith, University of KentuckyFollow

Start Date

10-17-2017 10:00 AM

Description

Biological Aspects:

Salamander (Ambystoma mexicanum) has a gigantic genome: ~32,000,000,000 bases (10X of size of human genome)

Sex is determined by a pair of morphologically identical chromosomes:

• ZZ in male
• ZW in female

Object:

• Find (if there are any) genomic differences between chromosomes W and Z

Workflow:

1. Sequencing and de novo assembly of the reference salamander genome
2. Alignment of short sequences from male and female genomes to the reference
3. Coverage analysis