Author ORCID Identifier

https://orcid.org/0000-0002-2002-0522

Date Available

11-20-2027

Year of Publication

2025

Document Type

Doctoral Dissertation

Degree Name

Doctor of Philosophy (PhD)

College

Arts and Sciences

Department/School/Program

Biology

Faculty

Dr. Bruce F. O'Hara

Faculty

Dr. Lauren N. Whitehurst

Faculty

Dr. Jakub Famulski

Abstract

Sleep is essential for health and survival, yet 30-45% of adults in the US regularly fail to obtain recommended sleep. Although the restorative functions of sleep are increasingly well characterized, the biological mechanisms through which sleep exerts these effects remain incompletely understood. The autonomic nervous system, comprising sympathetic and parasympathetic branches, shows marked stage-dependent fluctuations across the sleep cycle. Parasympathetic activity dominates during non-rapid eye movement sleep, whereas rapid eye movement sleep is accompanied by phasic sympathetic bursts. These changes have long been viewed as passive byproducts of central sleep processes, but the causal impact of ascending autonomic signals on human sleep regulation remains unclear. The dissertation tested whether transcutaneous vagus nerve stimulation, a noninvasive method that activates auricular vagal afferents and engages sleep–wake regulatory brainstem nuclei, can alter sleep architecture. Across two randomized, sham-controlled, within-subject studies (N = 92), participants received 90 minutes of stimulation during either early sleep (following stable N2; Study 1) or late sleep (at 3:30 AM; Study 2). Early-night stimulation increased N2 sleep and reduced REM percentage in men, and also increased spindle density. Together, these results suggest that afferent autonomic input can influence sleep regulation.

Digital Object Identifier (DOI)

https://doi.org/10.13023/etd.2025.529

Available for download on Saturday, November 20, 2027

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