US Patent Number
5646009
Publication Date
7-8-1997
Abstract
A hybrid vector carrying a first and second DNA segments operationally linked thereto, the first DNA segment encoding a protein capable of cross-linking to the cap structure of mRNA and mediating ribosome-binding, and the second DNA segment encoding a polypeptide or protein, the vector being capable of replication, transcription and translation to express the factor and the polypeptide or protein upon transformation of a eukaryotic host, and the polypeptide or protein being expressed at a level higher than the level of expression thereof in the absence of the first DNA segment. A eukaryotic host is transformed with this hybrid vector. Also disclosed is a method of increasing the synthesis of a polypeptide or protein in a eukaryotic host cell.
Assignees
University of Kentucky Research Foundation, Lexington, Ky.
Application Number
184,632
Filing Date
01/19/1994
Recommended Citation
Rhoads, Robert E. and Benedetti, Arrico De, "Hybrid Vector and Method Resulting in Protein Overproduction by Eukaryotic Cells" (1997). Molecular and Cellular Biochemistry Faculty Patents. 18.
https://uknowledge.uky.edu/biochem_patents/18
