Dysregulation of Wnt signaling is a hallmark of many cancers, and the development of effective, non-toxic small-molecule Wnt inhibitors is desirable. Off-target toxicities of new compounds are typically tested in mouse models, which is both costly and time consuming. Here, we present a rapid and inexpensive protocol to determine the in vivo toxicity and efficacy of novel Wnt inhibitors in zebrafish using a combination of a fluorescence reporter assay as well as eye rescue and fin regeneration assays. These experiments are completed within 1 week to rapidly narrow drug candidates before moving to more expensive pre-clinical testing.

For complete details on the use and execution of this protocol, please refer to Zhang et al. (2020).

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Notes/Citation Information

Published in STAR Protocols, v. 2, issue 2, 100433.

© 2021 The Authors

This is an open access article under the CC BY-NC-ND license (https://creativecommons.org/licenses/by-nc-nd/4.0/).

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Funding Information

This research was funded by a V Foundation V Scholar Award and NIH Grant DP2CA228043 (to J.S.B.) and NIH Training Grant T32CA165990 (to M.G.H.).

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