Dysregulation of Wnt signaling is a hallmark of many cancers, and the development of effective, non-toxic small-molecule Wnt inhibitors is desirable. Off-target toxicities of new compounds are typically tested in mouse models, which is both costly and time consuming. Here, we present a rapid and inexpensive protocol to determine the in vivo toxicity and efficacy of novel Wnt inhibitors in zebrafish using a combination of a fluorescence reporter assay as well as eye rescue and fin regeneration assays. These experiments are completed within 1 week to rapidly narrow drug candidates before moving to more expensive pre-clinical testing.
For complete details on the use and execution of this protocol, please refer to Zhang et al. (2020).
Digital Object Identifier (DOI)
This research was funded by a V Foundation V Scholar Award and NIH Grant DP2CA228043 (to J.S.B.) and NIH Training Grant T32CA165990 (to M.G.H.).
Haney, Meghan G.; Wimsett, Mary; Liu, Chunming; and Blackburn, Jessica S., "Protocol for Rapid Assessment of the Efficacy of Novel Wnt Inhibitors Using Zebrafish Models" (2021). Molecular and Cellular Biochemistry Faculty Publications. 183.