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Abstract
Dysregulation of Wnt signaling is a hallmark of many cancers, and the development of effective, non-toxic small-molecule Wnt inhibitors is desirable. Off-target toxicities of new compounds are typically tested in mouse models, which is both costly and time consuming. Here, we present a rapid and inexpensive protocol to determine the in vivo toxicity and efficacy of novel Wnt inhibitors in zebrafish using a combination of a fluorescence reporter assay as well as eye rescue and fin regeneration assays. These experiments are completed within 1 week to rapidly narrow drug candidates before moving to more expensive pre-clinical testing.
For complete details on the use and execution of this protocol, please refer to Zhang et al. (2020).
Document Type
Article
Publication Date
4-1-2021
Digital Object Identifier (DOI)
https://doi.org/10.1016/j.xpro.2021.100433
Funding Information
This research was funded by a V Foundation V Scholar Award and NIH Grant DP2CA228043 (to J.S.B.) and NIH Training Grant T32CA165990 (to M.G.H.).
Repository Citation
Haney, Meghan G.; Wimsett, Mary; Liu, Chunming; and Blackburn, Jessica S., "Protocol for Rapid Assessment of the Efficacy of Novel Wnt Inhibitors Using Zebrafish Models" (2021). Molecular and Cellular Biochemistry Faculty Publications. 183.
https://uknowledge.uky.edu/biochem_facpub/183
Graphical abstract
Notes/Citation Information
Published in STAR Protocols, v. 2, issue 2, 100433.
© 2021 The Authors
This is an open access article under the CC BY-NC-ND license (https://creativecommons.org/licenses/by-nc-nd/4.0/).