Microarray Dataset of Transient and Permanent DNA Methylation Changes in HeLa Cells Undergoing Inorganic Arsenic-Mediated Epithelial-to-Mesenchymal Transition

Authors

Meredith Eckstein, University of KentuckyFollow
Matthew Rea, University of KentuckyFollow
Yvonne N. Fondufe-Mittendorf, University of KentuckyFollow

Abstract

The novel dataset presented here represents the results of the changing pattern of DNA methylation profiles in HeLa cells exposed to chronic low dose (0.5 µM) sodium arsenite, resulting in epithelial-to-mesenchymal transition, as well as DNA methylation patterns in cells where inorganic arsenic has been removed. Inorganic arsenic is a known carcinogen, though not mutagenic. Several mechanisms have been proposed as to how inorganic arsenic drives carcinogenesis such as regulation of the cell׳s redox potential and/or epigenetics. In fact, there are gene specific studies and limited genome-wide studies that have implicated epigenetic factors such as DNA methylation in inorganic arsenic-mediated epithelial-to-mesenchymal transition (EMT). However, genome-wide studies about the impact of 1) chronic, low-dose inorganic arsenic exposure on DNA methylation patterns during inorganic arsenic-induced epithelial-to-mesenchymal transition, and 2) the removal inorganic arsenic (reversal) on DNA methylation patterns, is lacking. For this dataset, two replicates were performed with each of the samples – non-treated, inorganic arsenic-treated, and reverse-treated cells. We provide normalized and processed data, and log2 fold change in DNA methylation. The raw microarray data are available through NCBI GEO, accession number GSE95232 and a related research paper has been accepted for published in Toxicology and Applied Pharmacology (Eckstein et al., 2017) [1].

Document Type

Article

Publication Date

5-10-2017

Notes/Citation Information

Published in Data in Brief, v. 13, p. 6-9.

© 2017 The Author(s). Published by Elsevier Inc.

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

Digital Object Identifier (DOI)

https://doi.org/10.1016/j.dib.2017.05.002

Funding Information

This work was supported by NSF Grant MCB 1517986 to YFN-M, NIEHS Grant R01-ES024478 with diversity supplement 02S1 to YNF-M and NIH T32 Grant 165990 to MR, through Markey Cancer Center at University of Kentucky.

Related Content

Transparency data associated with this article can be found in the online version at https://doi.org/10.1016/j.dib.2017.05.002.

Supplementary data associated with this article can be found in the online version at https://doi.org/10.1016/j.dib.2017.05.002.

Repository Citation

Eckstein, Meredith; Rea, Matthew; and Fondufe-Mittendorf, Yvonne N., "Microarray Dataset of Transient and Permanent DNA Methylation Changes in HeLa Cells Undergoing Inorganic Arsenic-Mediated Epithelial-to-Mesenchymal Transition" (2017). Molecular and Cellular Biochemistry Faculty Publications. 122.
https://uknowledge.uky.edu/biochem_facpub/122