Date Available


Year of Publication


Degree Name

Master of Science in Biosystems and Agricultural Engineering (MSBiosyAgE)

Document Type

Master's Thesis


Agriculture; Engineering


Biosystems and Agricultural Engineering

First Advisor

Dr. Czarena Crofcheck


In order to utilize algae to fix CO2 and reduce Greenhouse gas emissions, large-scale, year-round algae cultivation will be required. This will require alga species that thrive in colder weather with media recipes based on commercially available fertilizers. The objectives of this study were to evaluate the effect of using commercial based media and the effect of pH when growing the cold-temperature algae Chloromonas rosae var. psychrophila. Commercial grade media ingredients urea, potash, triple superphosphate, and Sprint 330® were used to provide nitrogen, potassium, phosphorus, and EDTA with iron, respectively. The commercial grade media supported growth similar to the growth found with the lab-based media typically used. Additionally, tests were done to determine the effect of the pH of the media. Testing with initial pH targets of 5, 6, 7 and 8 using the laboratory media, showed that growth was not sufficient at a pH around 8. However both the laboratory and commercial grade media performed well when the initial pH was between 5 and 7, and no significant difference in algal growth was measured within this pH range.