Abstract
Ergot alkaloids, in their active isomeric form, affect animal health and performance, and adsorbents are used to mitigate toxicities by reducing bioavailability. Adsorbents with high specificity (molecularly imprinted polymers: MIP) adsorb ergot alkaloids in vitro, but require evaluation for biological implications. Using ex vivo myography, synthetic polymers were evaluated for effects on the bioactivity of ergotamine tartrate (ETA). Polymers were first evaluated using isotherms. Lateral saphenous veins were collected from 17 steers for four independent studies: dose response of ETA, adsorbent dose response, validation of pre-myograph incubation conditions and MIP/ non-molecularly imprinted polymer (NIP) comparison. Norepinephrine normalized percent contractile response to increasing ETA exhibited a sigmoidal dose response (max: 88.47 and log of the effective molar concentration (EC50) (−log [ETA]) of 6.66 ± 0.17 M). Although sample preparation time affected contractile response (p < 0.001), pre-myograph incubation temperature (39 vs. 21 °C, 1 h) had no effect (p > 0.05). Isothermal adsorption showed a maximum adsorption of 3.27E-008 moles·mg−1 and affinity between 0.51 and 0.57 mg (R2: 0.83–0.92) for both polymers, with no significant difference between polymers (p > 0.05). No significant differences in maximum inhibitory (p = 0.96) and IC50 responses (p = 0.163) between MIP and NIP were noticed. Normalized percent contraction could be predicted from the in vitro adsorption data (R2 = 0.87, p< 0.01), for both polymers. These studies indicate that synthetic polymers are potentially effective adsorbents to mitigate ergot toxicity caused by ergot alkaloids, with little evidence of significant differences between MIP and NIP in aqueous media.
Document Type
Article
Publication Date
2-2018
Digital Object Identifier (DOI)
https://doi.org/10.3390/toxins10020058
Funding Information
Funding of the study was sponsored by Alltech Incorporated, Nicholasville KY, USA.
Related Content
This is publication No. 17-07-119 of the Kentucky Agricultural Experiment Station and is published with the approval of the Director.
Repository Citation
Kudupoje, Manoj B.; Klotz, James L.; Yiannikouris, Alexandros; Dawson, Karl A.; McLeod, Kyle R.; and Vanzant, Eric S., "Contractile Response of Bovine Lateral Saphenous Vein to Ergotamine Tartrate Exposed to Different Concentrations of Molecularly Imprinted Polymer" (2018). Animal and Food Sciences Faculty Publications. 22.
https://uknowledge.uky.edu/animalsci_facpub/22
Included in
Beef Science Commons, Food Chemistry Commons, Molecular Biology Commons, Toxicology Commons
Notes/Citation Information
Published in Toxins, v. 10, issue 2, 58, p. 1-16.
© 2018 by the authors.
This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).