Predominant Expression of Alzheimer’s Disease-Associated BIN1 in Mature Oligodendrocytes and Localization to White Matter Tracts

Authors

Pierre De Rossi, University of Chicago
Virginie Buggia-Prévot, University of Chicago
Benjamin L. L. Clayton, University of Chicago
Jared B. Vasquez, University of KentuckyFollow
Carson van Sanford, University of Kentucky
Robert J. Andrew, University of Chicago
Ruben Lesnick, University of Chicago
Alexandra Botté, University of Chicago
Carole Deyts, University of Chicago
Someya Salem, University of Chicago
Eshaan Rao, University of Chicago
Richard C. Rice, University of Chicago
Angèle Parent, University of Chicago
Satyabrata Kar, University of Alberta, Canada
Brian Popko, University of Chicago
Peter Pytel, University of Chicago
Steven Estus, University of KentuckyFollow
Gopal Thinakaran, University of Chicago

Abstract

Background: Genome-wide association studies have identified BIN1 within the second most significant susceptibility locus in late-onset Alzheimer’s disease (AD). BIN1 undergoes complex alternative splicing to generate multiple isoforms with diverse functions in multiple cellular processes including endocytosis and membrane remodeling. An increase in BIN1 expression in AD and an interaction between BIN1 and Tau have been reported. However, disparate descriptions of BIN1 expression and localization in the brain previously reported in the literature and the lack of clarity on brain BIN1 isoforms present formidable challenges to our understanding of how genetic variants in BIN1 increase the risk for AD.

Methods: In this study, we analyzed BIN1 mRNA and protein levels in human brain samples from individuals with or without AD. In addition, we characterized the BIN1 expression and isoform diversity in human and rodent tissue by immunohistochemistry and immunoblotting using a panel of BIN1 antibodies.

Results: Here, we report on BIN1 isoform diversity in the human brain and document alterations in the levels of select BIN1 isoforms in individuals with AD. In addition, we report striking BIN1 localization to white matter tracts in rodent and the human brain, and document that the large majority of BIN1 is expressed in mature oligodendrocytes whereas neuronal BIN1 represents a minor fraction. This predominant non-neuronal BIN1 localization contrasts with the strict neuronal expression and presynaptic localization of the BIN1 paralog, Amphiphysin 1. We also observe upregulation of BIN1 at the onset of postnatal myelination in the brain and during differentiation of cultured oligodendrocytes. Finally, we document that the loss of BIN1 significantly correlates with the extent of demyelination in multiple sclerosis lesions.

Conclusion: Our study provides new insights into the brain distribution and cellular expression of an important risk factor associated with late-onset AD. We propose that efforts to define how genetic variants in BIN1 elevate the risk for AD would behoove to consider BIN1 function in the context of its main expression in mature oligodendrocytes and the potential for a role of BIN1 in the membrane remodeling that accompanies the process of myelination.

Document Type

Article

Publication Date

8-3-2016

Notes/Citation Information

Published in Molecular Neurodegeneration, v. 11, 59, p. 1-21.

© The Author(s). 2016

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

Digital Object Identifier (DOI)

https://doi.org/10.1186/s13024-016-0124-1

Funding Information

This study was supported by Cure Alzheimer’s Fund (GT), National Multiple Sclerosis Society and Illinois Department of Public Health Multiple Sclerosis Research Fund Grant (GT), BrightFocus Foundation (SE), and National Institutes of Health grants AG019070 (GT), AG045775 (SE), and NS034939 (PB). P.D.R. was supported by an AD Research fellowship from the Illinois Department of Public Health and V.B.P was supported by a postdoctoral fellowship from BrightFocus Foundation. C.V.S. was supported by the National Center for Advancing Translational Sciences grant UL1TR000117. The authors acknowledge the University of Kentucky Alzheimer’s Center (supported by P30-AG028383) for human tissue samples. Confocal imaging was performed at the Integrated Microscopy Core Facility at the University of Chicago (supported by S10OD010649). The use of University of Chicago’s Core Facilities was supported by the National Center For Advancing Translational Sciences Award 5 UL1 TR 000430–09.

Repository Citation

De Rossi, Pierre; Buggia-Prévot, Virginie; Clayton, Benjamin L. L.; Vasquez, Jared B.; van Sanford, Carson; Andrew, Robert J.; Lesnick, Ruben; Botté, Alexandra; Deyts, Carole; Salem, Someya; Rao, Eshaan; Rice, Richard C.; Parent, Angèle; Kar, Satyabrata; Popko, Brian; Pytel, Peter; Estus, Steven; and Thinakaran, Gopal, "Predominant Expression of Alzheimer’s Disease-Associated BIN1 in Mature Oligodendrocytes and Localization to White Matter Tracts" (2016). Physiology Faculty Publications. 83.
https://uknowledge.uky.edu/physiology_facpub/83