Abstract
Background: An HPLC method employing a post-column derivatization strategy using the cupric reducing antioxidant capacity reagent (CUPRAC reagent) for the determining antioxidants in plant-based materials leverages the separation capability of regular HPLC approaches while allowing for detection specificity for antioxidants.
Methods: Three different column types, namely core-shell and porous silica including two chemically different core-shell materials (namely phenyl-hexyl and C18), were evaluated to assess potential improvements that could be attained by changing from a porous silica matrix to a core-shell matrix. Tea extracts were used as sample matrices for the evaluation specifically looking at catechin and epigallocatechin gallate (EGCG).
Results: Both the C18 and phenyl-hexyl core-shell columns showed better performance compared to the C18 porous silica one in terms of separation, peak shape, and retention time. Among the two core-shell materials, the phenyl-hexyl column showed better resolving power compared to the C18 column.
Conclusions: The CUPRAC post-column derivatization method can be improved using core-shell columns and suitable for quantifying antioxidants, exemplified by catechin and EGCG, in tea samples.
Document Type
Article
Publication Date
1-17-2018
Digital Object Identifier (DOI)
https://doi.org/10.1186/s40543-018-0137-1
Funding Information
This study was made possible, in part, through funds from the Food and Drug Administration through grant RFA-FD-14-001.
Repository Citation
Haque, Syed A. and Cañete, Socrates Jose P., "HPLC-CUPRAC Post-Column Derivatization Method for the Determination of Antioxidants: A Performance Comparison Between Porous Silica and Core-Shell Column Packing" (2018). Kentucky Tobacco Research and Development Center Faculty Publications. 21.
https://uknowledge.uky.edu/ktrdc_facpub/21
Notes/Citation Information
Published in Journal of Analytical Science and Technology, v. 9, 4, p. 1-9.
© The Author(s). 2018
This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.