Publication Date
1997
Description
The objective of this study is to generate transgenic ryegrass plants with a down-regulation of two major pollen allergenic proteins Lol p I and Lol p II. Antisense vectors for Lol p I and Lol p II cDNAs driven by the pollen-specific promoter Zm13 have been constructed. Corresponding transgenic plants have been recovered by particle inflow gun-mediated microprojectile bombardment of embryogenic suspension cells from perennial and Italian ryegrass using chimeric Act1-hph gene as a selectable marker. Their transgenic nature has been demonstrated by Southern hybridization analysis. The analysis of reproductive development and accumulation of pollen allergens in the transgenic ryegrasses is in progress.
Citation
Wu, X L.; Ye, X D.; Wang, Z Y.; Potrykus, I; and Spangenberg, G, "Gene Transfer of Ryegrasses: Down-Regulation of Major Pollen Allergens in Transgenic Plants" (2024). IGC Proceedings (1997-2023). 18.
https://uknowledge.uky.edu/igc/1997/session13/18
Included in
Agricultural Science Commons, Agronomy and Crop Sciences Commons, Plant Biology Commons, Plant Pathology Commons, Soil Science Commons, Weed Science Commons
Gene Transfer of Ryegrasses: Down-Regulation of Major Pollen Allergens in Transgenic Plants
The objective of this study is to generate transgenic ryegrass plants with a down-regulation of two major pollen allergenic proteins Lol p I and Lol p II. Antisense vectors for Lol p I and Lol p II cDNAs driven by the pollen-specific promoter Zm13 have been constructed. Corresponding transgenic plants have been recovered by particle inflow gun-mediated microprojectile bombardment of embryogenic suspension cells from perennial and Italian ryegrass using chimeric Act1-hph gene as a selectable marker. Their transgenic nature has been demonstrated by Southern hybridization analysis. The analysis of reproductive development and accumulation of pollen allergens in the transgenic ryegrasses is in progress.
