Publication Date
1989
Description
Since it has been found that the plant regeneration of gramineous plants can be realized through somatic embryo genesis, a great development of tissue culture is obtained not only in cereals but also in grasses, which makes possible the genetic engineering and production of artificial seed. In addition, a progress has been made in selection of various valuable mutants by inducing calli or cells cultured to vary. For example, Zhou (1986) by inducing tobacco tissue on a medium containing 0.5 % NaCl has got NaCl-tolerant callus; then the callus were selected by increasing progressively the concentration of NaCl and finally regenerated NaCl-tolerant plantlets can live in 2 % NaCl. Tall wheatgrass (Agropyron elongatum) is a good forage having strong resistance to salinealkali. If it is used as original material, the getting of mutants with stronger resistivity is full of promise. For attaining the goal, it is necessary to realize plant regeneration from callus or cell. If the procedure as Zhou used is adapted, the first problem is to choose a proper explant and initial concentration of NaCl. For this reason, the presented paper shows only first step for inducing mutant in tall wheatgrass.
Citation
Decheng, Shi; Ti, Xi; and Tingcheng, Zhu, "Somatic Embryogenesis from Mature Embryo of Tall Wheatgrass (Agropyron elongatum) and Effects of NaCl on Induction and Growth of Callus from its Different Explants" (2025). IGC Proceedings (1989-2023). 73.
https://uknowledge.uky.edu/igc/1989/session3b/73
Included in
Agricultural Science Commons, Agronomy and Crop Sciences Commons, Plant Biology Commons, Plant Pathology Commons, Soil Science Commons, Weed Science Commons
Somatic Embryogenesis from Mature Embryo of Tall Wheatgrass (Agropyron elongatum) and Effects of NaCl on Induction and Growth of Callus from its Different Explants
Since it has been found that the plant regeneration of gramineous plants can be realized through somatic embryo genesis, a great development of tissue culture is obtained not only in cereals but also in grasses, which makes possible the genetic engineering and production of artificial seed. In addition, a progress has been made in selection of various valuable mutants by inducing calli or cells cultured to vary. For example, Zhou (1986) by inducing tobacco tissue on a medium containing 0.5 % NaCl has got NaCl-tolerant callus; then the callus were selected by increasing progressively the concentration of NaCl and finally regenerated NaCl-tolerant plantlets can live in 2 % NaCl. Tall wheatgrass (Agropyron elongatum) is a good forage having strong resistance to salinealkali. If it is used as original material, the getting of mutants with stronger resistivity is full of promise. For attaining the goal, it is necessary to realize plant regeneration from callus or cell. If the procedure as Zhou used is adapted, the first problem is to choose a proper explant and initial concentration of NaCl. For this reason, the presented paper shows only first step for inducing mutant in tall wheatgrass.
