Publication Date
1989
Description
Timothy (Phleum pratensis L.) is one of the most important grasses in Hokkaido, northern island of Japan, because of its winter hardiness and ability to give good quality hay. Recent advances in cellular and molecular biology of plant encourage to apply biotechnology not only to food crops but also to fodder crops (Vasil, 1985). To create new breeding materials, somaclonal variation, embryo rescue from wide cross and protoplast fusion have been studied extensively. In vitro propagation is re-emphasized as a basic requirement for all aspects of plant biotechnology in relation to agriculture. This serial studies have been carried out to establish methods aiming to get regenerated plants from timothy seeds callus. At the moment, plantlets are only obtained from immature spikes cultures (Akashi and Ikeda, 1987). At first these experiments were carried out to understand the culture conditions of callus induction, proliferation and regeneration on callus issued from timothy seeds.
Citation
Nakashima, H, "Tissue Culture of Timothy (Phleum pratensis L.)" (2025). IGC Proceedings (1989-2023). 69.
https://uknowledge.uky.edu/igc/1989/session3b/69
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Tissue Culture of Timothy (Phleum pratensis L.)
Timothy (Phleum pratensis L.) is one of the most important grasses in Hokkaido, northern island of Japan, because of its winter hardiness and ability to give good quality hay. Recent advances in cellular and molecular biology of plant encourage to apply biotechnology not only to food crops but also to fodder crops (Vasil, 1985). To create new breeding materials, somaclonal variation, embryo rescue from wide cross and protoplast fusion have been studied extensively. In vitro propagation is re-emphasized as a basic requirement for all aspects of plant biotechnology in relation to agriculture. This serial studies have been carried out to establish methods aiming to get regenerated plants from timothy seeds callus. At the moment, plantlets are only obtained from immature spikes cultures (Akashi and Ikeda, 1987). At first these experiments were carried out to understand the culture conditions of callus induction, proliferation and regeneration on callus issued from timothy seeds.