Publication Date
1989
Description
Plant transformation is a promising tool for plant breeding. It allows the introgression in the plant genome of alien genes. At the moment few, if any, genes of agronomical value have been cloned and are available for genetic engineering works ; on the contrary, some genes encoding for antibiotic resistance are available. The use of these genes allows an easy detection of transformation events and confers useful characters for in vitro selection. A direct application of antibiotic resistance is in the selection of hybrid colonies after protoplast fusion ofparentals resistant to different antibiotics. Transformation using Agrobacterium tumef aciens or Agrobacterium rhizogenes is at the moment one of the most efficient method for gene transfer in dicotyledonous species. Aim of this work is to set up the transformation procedures in different forage legumes using A. tumefaciens and A. rhizogenes derived binary vectors earring genes conferring resistance to different antibiotics.
Citation
Damiani, F; Pupilli, F; Arcioni, S; and Pezzotti, M, "Agrobacterium Mediated Transformation in Forage Legumes" (2025). IGC Proceedings (1989-2023). 64.
https://uknowledge.uky.edu/igc/1989/session3b/64
Included in
Agricultural Science Commons, Agronomy and Crop Sciences Commons, Plant Biology Commons, Plant Pathology Commons, Soil Science Commons, Weed Science Commons
Agrobacterium Mediated Transformation in Forage Legumes
Plant transformation is a promising tool for plant breeding. It allows the introgression in the plant genome of alien genes. At the moment few, if any, genes of agronomical value have been cloned and are available for genetic engineering works ; on the contrary, some genes encoding for antibiotic resistance are available. The use of these genes allows an easy detection of transformation events and confers useful characters for in vitro selection. A direct application of antibiotic resistance is in the selection of hybrid colonies after protoplast fusion ofparentals resistant to different antibiotics. Transformation using Agrobacterium tumef aciens or Agrobacterium rhizogenes is at the moment one of the most efficient method for gene transfer in dicotyledonous species. Aim of this work is to set up the transformation procedures in different forage legumes using A. tumefaciens and A. rhizogenes derived binary vectors earring genes conferring resistance to different antibiotics.
