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Publication Date

1977

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In vitro methods using rumen liquor for the determination of the digestibility of forages have the disadvantage of difficult use and restricted reproducibility. Therefore, the cel­lulolytic properties of the rumen inoculum were replaced by a commercial cellulase in order to get a simpler and more reproducible procedure. The values of in vivo digestibilities are reached if the cellulase digestion of feed samples is pretreated with dlilute hydrochloric acid and is followed by pepsin digestion. This cellulase digestibility alone or in combination with the contents of several crude nutrients can serve as parameter to estimate the energy value of feeds. The standard error of estima­tion is ± 5 per cent.

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On the estimation of digestibility and net energy in green forage and roughage by means of a cellulase method

In vitro methods using rumen liquor for the determination of the digestibility of forages have the disadvantage of difficult use and restricted reproducibility. Therefore, the cel­lulolytic properties of the rumen inoculum were replaced by a commercial cellulase in order to get a simpler and more reproducible procedure. The values of in vivo digestibilities are reached if the cellulase digestion of feed samples is pretreated with dlilute hydrochloric acid and is followed by pepsin digestion. This cellulase digestibility alone or in combination with the contents of several crude nutrients can serve as parameter to estimate the energy value of feeds. The standard error of estima­tion is ± 5 per cent.