The miR-15/107 family comprises a group of 10 paralogous microRNAs (miRNAs), sharing a 5' AGCAGC sequence. These miRNAs have overlapping targets. In order to characterize the expression of miR-15/107 family miRNAs, we employed customized TaqMan Low-Density micro-fluid PCR-array to investigate the expression of miR-15/107 family members, and other selected miRNAs, in 11 human tissues obtained at autopsy including the cerebral cortex, frontal cortex, primary visual cortex, thalamus, heart, lung, liver, kidney, spleen, stomach and skeletal muscle. miR-103, miR-195 and miR-497 were expressed at similar levels across various tissues, whereas miR-107 is enriched in brain samples. We also examined the expression patterns of evolutionarily conserved miR-15/107 miRNAs in three distinct primary rat brain cell preparations (enriched for cortical neurons, astrocytes and microglia, respectively). In primary cultures of rat brain cells, several members of the miR-15/107 family are enriched in neurons compared to other cell types in the central nervous system (CNS). In addition to mature miRNAs, we also examined the expression of precursors (pri-miRNAs). Our data suggested a generally poor correlation between the expression of mature miRNAs and their precursors. In summary, we provide a detailed study of the tissue and cell type-specific expression profile of this highly expressed and phylogenetically conserved family of miRNA genes.
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This study was supported by the National Institutes of Health, USA (Grant Nos. AG042419, NS085830 and AG028 383).
Supplementary data associated with this article can be found, in the online version, at http://dx.doi.org/10.1016/j.gpb.2013.10.003.
Wang, Wang-Xia; Danaher, Robert J.; Miller, Craig S.; Berger, Joseph R.; Nubia, Vega G.; Wilfred, Bernard R.; Neltner, Janna H.; Norris, Christopher M.; and Nelson, Peter T., "Expression of miR-15/107 Family MicroRNAs in Human Tissues and Cultured Rat Brain Cells" (2014). Sanders-Brown Center on Aging Faculty Publications. 97.
Figure S1. Correlation of expression between mature miRNAs and their precursors in human tissues.
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Table S1. List of miRNA probes on the TLDA cards.
1-s2.0-S1672022914000035-mmc3.doc (30 kB)
Table S2. Demographic information on the human autopsied cases examined in the present study.
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Table S3. Expression of mature miRNAs across various human tissues.
1-s2.0-S1672022914000035-mmc5.doc (41 kB)
Table S4. Ct values of miRNA precursor expression across various human tissues.
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Table S5. Ct values of mature miRNA expression across various human tissues.
1-s2.0-S1672022914000035-mmc7.doc (51 kB)
Table S6. Expression of mature and precursor miR-29 members in various human tissues.