Cranial radiation is important for treating both primary brain tumors and brain metastases. A potential delayed side effect of cranial radiation is neurocognitive function decline. Early detection of CNS injury might prevent further neuronal damage. Extracellular vesicles (EVs) have emerged as a potential diagnostic tool because of their unique membranous characteristics and cargos. We investigated whether EVs can be an early indicator of CNS injury by giving C57BJ/6 mice 10 Gy cranial IR. EVs were isolated from sera to quantify: 1) number of EVs using nanoparticle tracking analysis (NTA); 2) Glial fibrillary acidic protein (GFAP), an astrocyte marker; and 3) protein-bound 4-hydroxy-2-nonenal (HNE) adducts, an oxidative damage marker. Brain tissues were prepared for immunohistochemistry staining and protein immunoblotting. The results demonstrate: 1) increased GFAP levels (p < 0.05) in EVs, but not brain tissue, in the IR group; and 2) increased HNE-bound protein adduction levels (p < 0.05). The results support using EVs as an early indicator of cancer therapy-induced neuronal injury.
Digital Object Identifier (DOI)
Sukati, Suriyan; Ho, Jenni; Chaiswing, Luksana; Sompol, Pradoldej; Pandit, Harshul Shreekant; Wei, Wendy; Izumi, Tadahide; Chen, Quan; Weiss, Heidi; Noel, Teresa; Bondada, Subbarao; Butterfield, D. Allan; and St. Clair, Daret K., "Extracellular Vesicles Released After Cranial Radiation: An Insight into an Early Mechanism of Brain Injury" (2022). Sanders-Brown Center on Aging Faculty Publications. 194.