This is the first utilization of advanced analytical electron microscopy methods, including high-resolution transmission electron microscopy, high-angle annular dark field scanning transmission electron microscopy, electron energy loss spectroscopy, and energy-dispersive X-ray spectroscopy mapping to characterize the organ-specific bioprocessing of a relatively inert nanomaterial (nanoceria). Liver and spleen samples from rats given a single intravenous infusion of nanoceria were obtained after prolonged (90 days) in vivo exposure. These advanced analytical electron microscopy methods were applied to elucidate the organ-specific cellular and subcellular fate of nanoceria after its uptake. Nanoceria is bioprocessed differently in the spleen than in the liver.

Document Type


Publication Date


Notes/Citation Information

Published in Toxicologic Pathology, v. 46, issue 1, p. 47-61.

© The Author(s) 2017

The copyright holder has granted the permission for posting the article here.

The document available for download is the authors' post-peer-review final draft of the article.

Digital Object Identifier (DOI)


Funding Information

The author(s) disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: Research reported in this publication was supported by U.S. Environmental Protection Agency Science to Achieve Results (grant number RD-833772) and the National Institute of General Medical Sciences of the National Institutes of Health under Award Number R01GM109195.

Related Content

Supplementary material for this article is available online and also downloadable as the additional file shown at the end of this record.

Streaming Media

NIHMS909357-supplement-HRSTEM_Video.avi (339857 kB)
Supplemental file: HRSTEM Video (mp4 format)