This is the first utilization of advanced analytical electron microscopy methods, including high-resolution transmission electron microscopy, high-angle annular dark field scanning transmission electron microscopy, electron energy loss spectroscopy, and energy-dispersive X-ray spectroscopy mapping to characterize the organ-specific bioprocessing of a relatively inert nanomaterial (nanoceria). Liver and spleen samples from rats given a single intravenous infusion of nanoceria were obtained after prolonged (90 days) in vivo exposure. These advanced analytical electron microscopy methods were applied to elucidate the organ-specific cellular and subcellular fate of nanoceria after its uptake. Nanoceria is bioprocessed differently in the spleen than in the liver.
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The author(s) disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: Research reported in this publication was supported by U.S. Environmental Protection Agency Science to Achieve Results (grant number RD-833772) and the National Institute of General Medical Sciences of the National Institutes of Health under Award Number R01GM109195.
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Graham, Uschi M.; Yokel, Robert A.; Dozier, Alan K.; Drummy, Lawrence; Mahalingam, Krishnamurthy; Tseng, Michael T.; Birch, Eileen; and Fernback, Joseph, "Analytical High-Resolution Electron Microscopy Reveals Organ-Specific Nanoceria Bioprocessing" (2018). Pharmaceutical Sciences Faculty Publications. 109.