Loop Dynamics of Thymidine Diphosphate-Rhamnose 3'-O-Methyltransferase (CalS11), an Enzyme in Calicheamicin Biosynthesis

Authors

Lu Han, Rice University
Shanteri Singh, University of KentuckyFollow
Jon S. Thorson, University of KentuckyFollow
George N. Phillips Jr., Rice University

Abstract

Structure analysis and ensemble refinement of the apo-structure of thymidine diphosphate (TDP)-rhamnose 3'-O-methyltransferase reveal a gate for substrate entry and product release. TDP-rhamnose 3'-O-methyltransferase (CalS11) catalyses a 3'-O-methylation of TDP-rhamnose, an intermediate in the biosynthesis of enediyne antitumor antibiotic calicheamicin. CalS11 operates at the sugar nucleotide stage prior to glycosylation step. Here, we present the crystal structure of the apo form of CalS11 at 1.89 Å resolution. We propose that the L2 loop functions as a gate facilitating and/or providing specificity for substrate entry or promoting product release. Ensemble refinement analysis slightly improves the crystallographic refinement statistics and furthermore provides a compelling way to visualize the dynamic model of loop L2, supporting the understanding of its proposed role in catalysis.

Document Type

Article

Publication Date

2-2016

Notes/Citation Information

Published in Structural Dynamics, v. 3, no. 1, 012004, p. 1-8.

© 2016 Author(s).

All article content, except where otherwise noted, is licensed under a Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

Digital Object Identifier (DOI)

https://doi.org/10.1063/1.4941368

Funding Information 

This research was supported in part by National Institutes of Health Grant Nos. CA84374 (J.S.T.) and GM109456 (G.N.P.), National Institutes of Health Protein Structure Initiative Grant Nos. U01 GM098248 and NSF 1231306 (BioXFEL). Use of the LS-CAT Sector 21 was supported by the Michigan Economic Development Corporation and the Michigan Technology Tri-Corridor for the support of this research program (Grant No. 085P1000817).

Repository Citation

Han, Lu; Singh, Shanteri; Thorson, Jon S.; and Phillips, George N. Jr., "Loop Dynamics of Thymidine Diphosphate-Rhamnose 3'-O-Methyltransferase (CalS11), an Enzyme in Calicheamicin Biosynthesis" (2016). Center for Pharmaceutical Research and Innovation Faculty Publications. 2.
https://uknowledge.uky.edu/cpri_facpub/2