Abstract
An important strategy in the construction of biomimetic membranes and devices is to use natural proteins as the functional components for incorporation in a polymeric or nanocomposite matrix. Toward this goal, an important step is to immobilize proteins with high efficiency and precision without disrupting the protein function. Here, we developed a dual-functional tag containing histidine and the non-natural amino acid azidohomoalanine (AHA). AHA is metabolically incorporated into the protein, taking advantage of the Met-tRNA and Met-tRNA synthetase. Histidine in the tag can facilitate metal-affinity purification, whereas AHA can react with an alkyne-functionalized probe or surface via well-established click chemistry. We tested the performance of the tag using two model proteins, green fluorescence protein and an enzyme pyrophosphatase. We found that the addition of the tag and the incorporation of AHA did not significantly impair the properties of these proteins, and the histidine–AHA tag can facilitate protein purification, immobilization, and labeling.
Document Type
Article
Publication Date
2-13-2017
Digital Object Identifier (DOI)
https://doi.org/10.1021/acsomega.6b00512
Funding Information
This work was supported by the Kentucky NSF EPSCoR RII Award 1355438 (to D.B. and Y.W.) and the Igniting Research Collaboration Award from the University of Kentucky (to Y.W.).
Repository Citation
Zhang, Xinyi; Lu, Wei; Kwan, Kevin; Bhattacharyya, Dibakar; and Wei, Yinan, "Dual-Functional-Tag-Facilitated Protein Labeling and Immobilization" (2017). Chemistry Faculty Publications. 96.
https://uknowledge.uky.edu/chemistry_facpub/96
Included in
Amino Acids, Peptides, and Proteins Commons, Cell and Developmental Biology Commons, Chemistry Commons, Molecular Biology Commons
Notes/Citation Information
Published in ACS Omega, v. 2, issue 2, p. 522-528.
Copyright © 2017 American Chemical Society
This is an open access article published under an ACS AuthorChoice License, which permits copying and redistribution of the article or any adaptations for non-commercial purposes.