Author ORCID Identifier

https://orcid.org/0000-0002-5830-5902

Year of Publication

2019

Degree Name

Master of Science (MS)

Document Type

Master's Thesis

College

Arts and Sciences

Department

Chemistry

First Advisor

Dr. Bert Lynn

Abstract

Exposomics is an emerging area of study that looks at how the environment around a person or persons affects their overall health. Biomarkers have emerged as useful tools to better understand how the exposome affects a person. In this work, we investigate two potential endogenous biomarkers, homocysteine and glutathione that have been previously implicated in a number of diseases that have been linked to environmental causes. We also investigated an environmental exposure, the fungicide Ziram, which epidemiologically has been linked to diseases. In our investigation, we utilized capillary electrophoresis and capillary electrophoresis-mass spectrometry to develop a method for homocysteine and identify a derivative to keep it from auto-oxidizing. We utilized mass spectrometry to identify the best ionization technique to detect Ziram and confirmation of where it binds on thiol-containing molecules. We also developed a method to extract glutathione-Ziram from serum and utilized liquid chromatography-mass spectrometry to begin a validation of glutathione-Ziram. Although future work is needed, we believe that this work was the beginning steps to understand biomarkers and their role in the exposome.

Digital Object Identifier (DOI)

https://doi.org/10.13023/etd.2020.032

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