Author ORCID Identifier

Date Available


Year of Publication


Degree Name

Master of Science in Biomedical Engineering

Document Type

Master's Thesis




Biomedical Engineering

First Advisor

Dr. Guigen Zhang


Biosensors and their use in both the research and clinical field for the detection and monitoring of critical biomarkers are prevalent and constantly improving. However, continued research needs to be done to address shortcomings, such as low sensitivity, poor specificity, and poor readiness for integration into research use and patient care. The objective of this research was to create a combined fluidic, chip-based biosensor that could detect different biomarkers with high sensitivity and ease of use. For assessing the developed sensor, three separate biomarkers were tested: glucose, cholesterol, and oxygen. Both the glucose biosensor and cholesterol biosensor were combined with the microfluidic platform for biomarker detection testing. The oxygen biosensor was tested as a stand-alone chip, with future work including the combination with the microfluidic platform. Results of stepwise, amperometric tests prove the success of the microfluidic, chip-based biosensor for both glucose and cholesterol detection within the respective physiological ranges, with the glucose biosensor showing high sensitivity and a low limit of detection. The oxygen biosensor also proved successful in detecting changes in oxygen concentration in solution within physiological ranges of arterial oxygen partial pressure.

Digital Object Identifier (DOI)

Funding Information

This research was supported by the F. Joseph Halcomb III, M.D. Endowed Fund.